Juvenile hormone regulates photoperiod-mediated male reproductive diapause via the methoprene-tolerant gene in the ladybeetle Harmonia axyridis,Insect Science

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Juvenile hormone regulates photoperiod-mediated male reproductive diapause via the methoprene-tolerant gene in the ladybeetle Harmonia axyridis
Insect Science ( IF 2.9 ) Pub Date : 2021-04-11 , DOI: 10.1111/1744-7917.12918
Qiao Gao ₁ , Bei Li ₁ , Bing-Xin Wei ₁ , Wen Liu ₁ , Pan Wang ₂ , Jia-Lu Wang ₁ , Xing-Miao Zhou ₁ , Xiao-Ping Wang ₁

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Juvenile hormone (JH) absence induces photoperiod-mediated reproductive diapause, which is characterized by reproductive cessation. Although the role of methoprene-tolerant (Met)-mediated JH signaling in photoperiod-mediated female reproduction has been well documented, its role in male reproduction remains unclear. In this study, we investigated the role of JH in regulating photoperiod-mediated development of the male internal reproductive system (IRS) in the predatory ladybeetle Harmonia axyridis (Pallas). In a previous study, we found that adult male H. axyridis reared under either a short-day (SD) or long-day (LD) photoperiod had obvious differences in IRS development, but we were unable to identify the regulators of male reproductive diapause. In this study, we found that beetles reared under an SD photoperiod had significantly lower JH titer and a relatively undeveloped male IRS compared with those reared under an LD photoperiod. Additionally, application of the JH analog (JHA) methoprene promoted IRS development. Furthermore, Met knockdown strongly blocked JH signaling in males reared under the LD photoperiod, thereby slowing IRS development. Moreover, exogenous JHA did not reverse the suppressed development of the male IRS caused by Met knockdown. These results indicate that photoperiod regulates male IRS development in H. axyridis through a conserved Met-dependent JH signaling pathway.

中文翻译：

保幼激素通过瓢虫异色瓢虫耐甲氧戊二烯基因调节光周期介导的雄性生殖滞育

保幼激素 (JH) 缺乏会导致光周期介导的生殖滞育，其特征是生殖停止。尽管甲氧戊二烯耐受 (Met) 介导的 JH 信号在光周期介导的雌性生殖中的作用已得到充分证明，但其在雄性生殖中的作用仍不清楚。在这项研究中，我们研究了 JH 在调节捕食性瓢虫异色瓢虫（Pallas）中雄性内部生殖系统 (IRS) 的光周期介导发育中的作用。在之前的一项研究中，我们发现成年雄性异色瓢虫在短日照 (SD) 或长日照 (LD) 光周期下饲养的 IRS 发育存在明显差异，但我们无法确定雄性生殖滞育的调节因子。在这项研究中，我们发现与在 LD 光周期下饲养的甲虫相比，在 SD 光周期下饲养的甲虫具有显着较低的 JH 滴度和相对不发达的雄性 IRS。此外，JH 类似物 (JHA) 甲氧普林的应用促进了 IRS 的发展。此外，Met敲低强烈阻断了在 LD 光周期下饲养的雄性的 JH 信号传导，从而减缓了 IRS 的发展。此外，外源性 JHA 并没有逆转由Met击倒引起的男性 IRS 抑制发展。这些结果表明光周期调节雄性 IRS 的发育H. axyridis通过保守的 Met 依赖性 JH 信号通路。

更新日期：2021-04-11

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