Random-pattern skin flap is widely used in tissue reconstruction. However, necrosis occurring in the distal part of the flap limits its clinical application to some extent. Activation of autophagy has been considered as an effective approach to enhance the survival of skin flaps. Pseudoginsenoside F11 (PF11), an ocotillol-type saponin, is an important component of Panax quinquefolium which has been shown to confer protection against cerebral ischemia and alleviate oxidative stress. However, it is currently unknown whether PF11 induces autophagy to improve the survival of skin flaps. In this study, we investigated the effects of PF11 on blood flow and tissue edema. The results of histological examination and western blotting showed that PF11 enhanced angiogenesis, alleviated apoptosis and oxidative stress, thereby improving the survival of the flap. Further experiments showed that PF11 promoted nuclear translocation of TFEB and by regulating the phosphorylation of AMPK. In summary, this study demonstrates that PF11 activates autophagy through AMPK-TFEB signal pathway in skin flaps and it could be a promising strategy for enhancing flap viability.

中文翻译：

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Pseudoginsenoside F11通过促进AMPEB-mTOR信号途径的TFEB核易位而增强了随机型皮瓣的生存能力

随机模式皮瓣广泛用于组织重建。但是，发生在皮瓣远端的坏死在一定程度上限制了其临床应用。自噬的激活被认为是提高皮瓣存活率的有效方法。伪皂苷皂苷F11（PF11）是一种Ocotillol型皂苷，是西洋参的重要组成部分，已被证明具有抗脑缺血作用和减轻氧化应激的作用。但是，目前尚不知道PF11是否诱导自噬以改善皮瓣的存活。在这项研究中，我们调查了PF11对血流量和组织水肿的影响。组织学检查和蛋白质印迹的结果表明，PF11增强了血管生成，减轻了细胞凋亡和氧化应激，从而提高了皮瓣的存活率。进一步的实验表明，PF11通过调节AMPK的磷酸化来促进TFEB的核易位。总而言之，这项研究表明PF11通过皮瓣中的AMPK-TFEB信号通路激活自噬，并且可能是增强皮瓣生存力的一种有前途的策略。