FleXert：用于拉伸细胞培养的柔软、可驱动的多孔板插入物,ACS Biomaterials Science & Engineering

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FleXert：用于拉伸细胞培养的柔软、可驱动的多孔板插入物
ACS Biomaterials Science & Engineering ( IF 5.4 ) Pub Date : 2021-04-12 , DOI: 10.1021/acsbiomaterials.0c01448
Sara Correia Carreira ₁ , Majid Taghavi ₂ , Elizabeth Pavez Loriè ₃ , Jonathan Rossiter ₂

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多孔多孔板插件广泛用于生物医学研究，以研究运输过程或在气液界面培养细胞/组织。这些插入物由刚性材料制成，并在静态培养条件下使用，不代表生物微环境。在这里，我们展示了 FleXert，这是一种与六孔板接口的柔软、可驱动的细胞培养插件。它由聚二甲基硅氧烷 (PDMS) 制成，包含多孔 PDMS 膜作为细胞/组织支持物。FleXerts 可以使用标准注射泵气动驱动，产生高达 30% 的拉伸应变。通过改变气压和泵速，可以实现多种驱动模式。具有纤连蛋白的 FleXert 多孔 PDMS 膜的轻松表面功能化能够粘附人皮肤成纤维细胞，并且在 FleXert 膜上发育的菌株成功转导到细胞层。3D 组织模型，例如载有成纤维细胞的胶原凝胶，也可以在聚多巴胺涂层后锚定到 PDMS。此外，胶原蛋白涂层的 FleXert 膜支持建立人体皮肤模型，证明该材料具有组织工程所需的出色生物相容性。与现有技术相比，FleXerts 不需要昂贵的制造设备或定制的培养室，使其成为生理应变下组织工程和生物屏障渗透研究的通用且低成本的解决方案。

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FleXert: A Soft, Actuatable Multiwell Plate Insert for Cell Culture under Stretch

Porous multiwell plate inserts are widely used in biomedical research to study transport processes or to culture cells/tissues at the air–liquid interface. These inserts are made of rigid materials and used under static culture conditions, which are unrepresentative of biological microenvironments. Here, we present FleXert, a soft, actuatable cell culture insert that interfaces with six-well plates. It is made of polydimethylsiloxane (PDMS) and comprises a porous PDMS membrane as cell/tissue support. FleXerts can be pneumatically actuated using a standard syringe pump, imparting tensile strains of up to 30%. A wide range of actuation patterns can be achieved by varying the air pressure and pumping rate. Facile surface functionalization of FleXert’s porous PDMS membrane with fibronectin enables adhesion of human dermal fibroblasts and strains developing on FleXert’s membrane are successfully transduced to the cell layer. 3D tissue models, such as fibroblast-laden collagen gels, can also be anchored to PDMS following polydopamine coating. Furthermore, collagen-coated FleXert membranes support the establishment of a human skin model, demonstrating the material’s excellent biocompatibility required for tissue engineering. In contrast to existing technologies, FleXerts do not require costly fabrication equipment or custom-built culture chambers, making them a versatile and low-cost solution for tissue engineering and biological barrier penetration studies under physiological strain. This paper is an extensive toolkit for multidisciplinary mechanobiology studies, including detailed instructions for a wide variety of methods such as device fabrication, theoretical modeling, cell culture, and image analysis techniques.

更新日期：2021-06-14

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