Alloimperatorin is a compound extracted from the traditional Chinese medicine (Angelica dahurica), which has exhibited anticancer activity. However, its precise molecular mechanism of anticancer remains unclear. Alloimperatorin-induced apoptosis of cervical cancer cells and its molecular mechanism were investigated in the present study. Cholecystokinin octapeptide (CCK-8) was employed to evaluate the cytotoxicity of alloimperatorin on HeLa, SiHa, and MS-751 cells. Flow cytometry was used to assess apoptosis induced by alloimperatorin. The mechanism of apoptosis was verified by mitochondrial membrane potential, Western blotting, and fluorescent PCR. The results of the study showed that alloimperatorin reduced the activity of HeLa cells. The calculated IC₅₀ at 48 hours was 116.9 μM. Compared with the control group, alloimperatorin increased the apoptotic rate of HeLa cells and reduced the mitochondrial membrane potential of HeLa cells. The Western blot results showed that alloimperatorin promotes the expression of caspase3, 8, 9 and that Bax apoptotic proteins reduce PARP expression, procaspase3, 8, 9, and BCL-2 proteins and reduces the cyt-c in the mitochondria expression. The results demonstrated that alloimperatorin can induce HeLa cell apoptosis through mitochondria and extrinsic apoptotic pathways.

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异源欧前胡素对HeLa细胞增殖和凋亡机制的研究

异欧前胡素是从具有抗癌活性的中药（当归）中提取的化合物。但是，其确切的抗癌分子机制仍不清楚。本研究研究了异顶递质诱导的宫颈癌细胞凋亡及其分子机制。胆囊收缩素八肽（CCK-8）被用来评估同素异黄酮对HeLa，SiHa和MS-751细胞的细胞毒性。流式细胞仪用于评估异欧前胡素诱导的细胞凋亡。通过线粒体膜电位，Western印迹和荧光PCR验证了凋亡的机制。研究结果表明，异欧前胡素降低了HeLa细胞的活性。所计算的IC ₅₀在48小时时是116.9  μM.与对照组相比，同素欧前胡素提高了HeLa细胞的凋亡率，并降低了HeLa细胞的线粒体膜电位。蛋白质印迹结果显示，异欧前胡素可促进caspase3、8、9的表达，而Bax凋亡蛋白可降低PARP，procaspase3、8、9和BCL-2的表达，并降低线粒体中cyt-c的表达。结果表明，异欧前胡素可通过线粒体和外在凋亡途径诱导HeLa细胞凋亡。