Alloimperatorin is a compound extracted from the traditional Chinese medicine (Angelica dahurica), which has exhibited anticancer activity. However, its precise molecular mechanism of anticancer remains unclear. Alloimperatorin-induced apoptosis of cervical cancer cells and its molecular mechanism were investigated in the present study. Cholecystokinin octapeptide (CCK-8) was employed to evaluate the cytotoxicity of alloimperatorin on HeLa, SiHa, and MS-751 cells. Flow cytometry was used to assess apoptosis induced by alloimperatorin. The mechanism of apoptosis was verified by mitochondrial membrane potential, Western blotting, and fluorescent PCR. The results of the study showed that alloimperatorin reduced the activity of HeLa cells. The calculated IC₅₀ at 48 hours was 116.9 μM. Compared with the control group, alloimperatorin increased the apoptotic rate of HeLa cells and reduced the mitochondrial membrane potential of HeLa cells. The Western blot results showed that alloimperatorin promotes the expression of caspase3, 8, 9 and that Bax apoptotic proteins reduce PARP expression, procaspase3, 8, 9, and BCL-2 proteins and reduces the cyt-c in the mitochondria expression. The results demonstrated that alloimperatorin can induce HeLa cell apoptosis through mitochondria and extrinsic apoptotic pathways.

中文翻译：

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别欧前胡素对HeLa细胞增殖和凋亡机制的研究


别欧前胡素是从中药（当归）中提取的化合物，具有抗癌活性。但其确切的抗癌分子机制仍不清楚。本研究探讨别欧前胡素诱导宫颈癌细胞凋亡及其分子机制。采用胆囊收缩素八肽 (CCK-8) 评估别欧前胡素对 HeLa、SiHa 和 MS-751 细胞的细胞毒性。使用流式细胞术评估别欧前胡素诱导的细胞凋亡。通过线粒体膜电位、Western blotting和荧光PCR验证了细胞凋亡的机制。研究结果表明，别欧前胡素降低了 HeLa 细胞的活性。计算48小时的IC ₅₀为116.9 μM 。与对照组相比，别欧前胡素增加了HeLa细胞的凋亡率，降低了HeLa细胞的线粒体膜电位。 Western blot结果显示，alloimperatorin促进caspase3、8、9的表达，Bax凋亡蛋白降低PARP、procaspase3、8、9和BCL-2蛋白的表达，降低线粒体中cyt-c的表达。结果表明，别欧前胡素可通过线粒体和外源性凋亡途径诱导HeLa细胞凋亡。