疏水BCAA肽的降血糖作用与PI3K / Akt蛋白表达的改变有关。,Journal of Agricultural and Food Chemistry

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疏水BCAA肽的降血糖作用与PI3K / Akt蛋白表达的改变有关。
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2021-04-06 , DOI: 10.1021/acs.jafc.1c00726
Xiping Zhu _{1,

2} , Wei Wang ₁ , Chun Cui _{1,

3}

Affiliation

在2型糖尿病db / db小鼠中初步表征了沙棘种子蛋白的疏水性支链氨基酸（BCAA）肽的降血糖活性。四种新颖的BCAA肽（18.27±0.26％（w / w）：Leu / Ile-Pro-Glu-Asp-Pro，Asp-Leu / Ile-Val-Gly-Glu，Leu / Ile-Pro和Leu / Ile-在沙棘种子蛋白中鉴定出Pro-Leu / Ile）。使用80％乙醇获得的沙棘种子蛋白质水解物中的蛋白质含量为78.8±1.4％（w / w）。动物实验表明，口服BCAA肽（全部四个）可显着逆转糖尿病症状。与db / db组（对照组）相比，用BCAA肽（0.5、1.0、2.0 mg /（gd））治疗后，体重和胰岛素抵抗得以改善。还，该治疗通过上调葡萄糖转运蛋白4（GULT4）显着降低了空腹血糖（FBG）水平。此外，BCAA肽通过下调蛋白激酶B（AKT）和糖原合酶激酶-3β（GSK-3β）显着增加了肌肉糖原含量（22.6±0.9 nmol / mg），同时增加了糖原合酶（GS）的活性。BCAA肽还显着上调了磷脂酰肌醇3-激酶（PI3K）的蛋白质水平。我们显示，BCAA肽减轻了与db / db小鼠骨骼肌中改变的PI3K / Akt蛋白表达相关的胰岛素抵抗。通过降低蛋白激酶B（AKT）和糖原合酶激酶3β（GSK-3β）的表达，同时增加糖原合酶（GS）的活性，可达到9 nmol / mg）。BCAA肽还显着上调了磷脂酰肌醇3-激酶（PI3K）的蛋白质水平。我们显示，BCAA肽减轻了与db / db小鼠骨骼肌中改变的PI3K / Akt蛋白表达相关的胰岛素抵抗。通过降低蛋白激酶B（AKT）和糖原合酶激酶3β（GSK-3β）的表达，同时增加糖原合酶（GS）的活性，可达到9 nmol / mg）。BCAA肽还显着上调了磷脂酰肌醇3-激酶（PI3K）的蛋白质水平。我们显示，BCAA肽减轻了与db / db小鼠骨骼肌中改变的PI3K / Akt蛋白表达相关的胰岛素抵抗。

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Hypoglycemic Effect of Hydrophobic BCAA Peptides Is Associated with Altered PI3K/Akt Protein Expression

The hypoglycemic activities of the hydrophobic branched-chain amino acid (BCAA) peptides from seabuckthorn seed protein were preliminarily characterized in type 2 diabetic db/db mice. Four novel BCAA peptides (18.27 ± 0.26% (w/w): Leu/Ile-Pro-Glu-Asp-Pro, Asp-Leu/Ile-Val-Gly-Glu, Leu/Ile-Pro, and Leu/Ile-Pro-Leu/Ile) were identified in seabuckthorn seed protein. The protein content in seabuckthorn seed protein hydrolysate, obtained using 80% ethanol, was 78.8 ± 1.4% (w/w). Animal experiments revealed that oral administration of BCAA peptides (all four) significantly reversed the diabetic symptoms. Compared to the db/db group (control), body weight and insulin resistance were ameliorated after treatment with BCAA peptides (0.5, 1.0, 2.0 mg/(g d)). Also, the treatment remarkably reduced the fasting blood glucose (FBG) levels by upregulation of glucose transporter 4 (GULT4). Moreover, BCAA peptides significantly increased the muscle glycogen content (22.6 ± 0.9 nmol/mg) via the downregulation of protein kinase B (AKT) and glycogen synthase kinase-3β (GSK-3β) while increasing the activity of glycogen synthase (GS). BCAA peptides also significantly upregulated the protein levels of phosphatidylinositol 3-kinase (PI3K). We show that BCAA peptides alleviated insulin resistance associated with altered PI3K/Akt protein expression in the skeletal muscle of db/db mice.

更新日期：2021-04-21

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