Bacillus licheniformis is widely used to produce various valuable products, such as food enzymes, industrial chemicals, and biocides. The carbon catabolite regulation process in the utilization of raw materials is crucial to maximizing the efficiency of this microbial cell factory. The current understanding of the molecular mechanism of this regulation is based on limited motif patterns in protein-DNA recognition, where the typical catabolite-responsive element (CRE) motif is “TGWNANCGNTNWCA”. Here, CRETre is identified and characterized as a new CRE. It consists of two palindrome arms of 6 nucleotides (AGCTTT/AAAGCT) and an intermediate spacer. CRETre is involved in bidirectional regulation in a glucose stress environment. When AGCTTT appears in the 5′ end, the regulatory element exhibits a carbon catabolite activation effect, while AAAGCT in the 5′ end corresponds to carbon catabolite repression. Further investigation indicated a wide occurrence of CRETre in the genome of B. licheniformis.

中文翻译：

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新的 CcpA 结合位点在地衣芽孢杆菌碳分解代谢中发挥双向作用


地衣芽孢杆菌广泛用于生产各种有价值的产品，如食品酶、工业化学品和杀菌剂。原材料利用中的碳分解代谢物调节过程对于最大限度地提高微生物细胞工厂的效率至关重要。目前对该调节分子机制的理解是基于蛋白质-DNA识别中的有限基序模式，其中典型的分解代谢物响应元件（CRE）基序是“TGWNANCGNTNWCA”。在这里，CRETre 被识别并表征为一种新的 CRE。它由两个 6 个核苷酸的回文臂 (AGCTTT/AAAGCT) 和一个中间间隔区组成。 CRETre 参与葡萄糖应激环境中的双向调节。当AGCTTT出现在5'端时，调节元件表现出碳分解代谢物激活作用，而AAAGCT出现在5'端则对应于碳分解代谢物抑制。进一步的研究表明 CRETre 在地衣芽孢杆菌基因组中广泛存在。