两种 ABC 转运系统在粪肠球菌中进行肽摄取：它们在生长和性信息素摄取中的作用,Molecular Microbiology

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两种 ABC 转运系统在粪肠球菌中进行肽摄取：它们在生长和性信息素摄取中的作用
Molecular Microbiology ( IF 2.6 ) Pub Date : 2021-04-05 , DOI: 10.1111/mmi.14725
Takaya Segawa ₁ , Christopher M Johnson ₁ , Ronnie P-A Berntsson _{2,

3} , Gary M Dunny ₁

Affiliation

肠球菌信息素诱导型质粒编码预测的 OppA 家族分泌的脂蛋白。在质粒 pCF10 的情况下，蛋白质是 PrgZ，它增强了对 cCF10 信息素的交配反应。OppA 蛋白通常与相关的 OppBCDF ABC 转运蛋白一起发挥作用以输入肽。在这项研究中，我们使用两种信息素诱导型荧光报告构建体分析了 PrgZ 与两种宿主编码的 Opp 转运蛋白的潜在相互作用。根据我们的结果，我们建议重命名这些基因座opp1 (OG1RF_10634-10639) 和opp2 (OG1RF_12366-12370)。我们还检查了 Opp1 和 Opp2 系统在没有 PrgZ 的情况下调解导入的能力。如果opp1或opp2表达 PrgZ 的细胞能够导入信息素是功能性的，但如果两个opp基因座都被破坏，则不会。在没有 PrgZ 的情况下，信息素的导入依赖于功能性opp2系统，包括opp2A。PrgZ、Opp1A、Opp2A 和相关乳酸乳球菌OppA 蛋白的肽结合口袋的比较结构分析表明，PrgZ 强大的信息素结合能力与疏水肽的近乎最佳拟合有关，而 Opp2A 的结合能力可能是由类似于L. lactis OppA 的更开放、混杂的肽结合口袋造成的。

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Two ABC transport systems carry out peptide uptake in Enterococcus faecalis: Their roles in growth and in uptake of sex pheromones

Enterococcal pheromone-inducible plasmids encode a predicted OppA-family secreted lipoprotein. In the case of plasmid pCF10, the protein is PrgZ, which enhances the mating response to cCF10 pheromone. OppA proteins generally function with associated OppBCDF ABC transporters to import peptides. In this study, we analyzed the potential interactions of PrgZ with two host-encoded Opp transporters using two pheromone-inducible fluorescent reporter constructs. Based on our results, we propose renaming these loci opp1 (OG1RF_10634-10639) and opp2 (OG1RF_12366-12370). We also examined the ability of the Opp1 and Opp2 systems to mediate import in the absence of PrgZ. Cells expressing PrgZ were able to import pheromone if either opp1 or opp2 was functional, but not if both opp loci were disrupted. In the absence of PrgZ, pheromone import was dependent on a functional opp2 system, including opp2A. Comparative structural analysis of the peptide-binding pockets of PrgZ, Opp1A, Opp2A, and the related Lactococcus lactis OppA protein, suggested that the robust pheromone-binding ability of PrgZ relates to a nearly optimal fit of the hydrophobic peptide, whereas binding ability of Opp2A likely results from a more open, promiscuous peptide-binding pocket similar to L. lactis OppA.

更新日期：2021-04-05

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