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Structure of plant photosystem I−light harvesting complex I supercomplex at 2.4 Å resolution
Journal of Integrative Plant Biology ( IF 9.3 ) Pub Date : 2021-03-31 , DOI: 10.1111/jipb.13095
Jie Wang 1, 2 , Long-Jiang Yu 1 , Wenda Wang 1 , Qiujing Yan 1, 2 , Tingyun Kuang 1 , Xiaochun Qin 3 , Jian-Ren Shen 1, 4
Affiliation  

Photosystem I (PSI) is one of the two photosystems in photosynthesis, and performs a series of electron transfer reactions leading to the reduction of ferredoxin. In higher plants, PSI is surrounded by four light-harvesting complex I (LHCI) subunits, which harvest and transfer energy efficiently to the PSI core. The crystal structure of PSI-LHCI supercomplex has been analyzed up to 2.6 Å resolution, providing much information on the arrangement of proteins and cofactors in this complicated supercomplex. Here we have optimized crystallization conditions, and analyzed the crystal structure of PSI-LHCI at 2.4 Å resolution. Our structure showed some shift of the LHCI, especially the Lhca4 subunit, away from the PSI core, suggesting the indirect connection and inefficiency of energy transfer from this Lhca subunit to the PSI core. We identified five new lipids in the structure, most of them are located in the gap region between the Lhca subunits and the PSI core. These lipid molecules may play important roles in binding of the Lhca subunits to the core, as well as in the assembly of the supercomplex. The present results thus provide novel information for the elucidation of the mechanisms for the light-energy harvesting, transfer and assembly of this supercomplex.

中文翻译:

植物光系统 I-光收集复合物 I 超复合物的结构,分辨率为 2.4 Å

光系统 I (PSI) 是光合作用中的两个光系统之一,执行一系列电子转移反应,导致铁氧还蛋白还原。在高等植物中,PSI 被四个光捕获复合体 I (LHCI) 亚基包围,这些亚基有效地收集能量并将其转移到 PSI 核心。PSI-LHCI 超复合物的晶体结构分析分辨率高达 2.6 Å,提供了有关该复杂超复合物中蛋白质和辅因子排列的大量信息。在这里,我们优化了结晶条件,并以 2.4 Å 的分辨率分析了 PSI-LHCI 的晶体结构。我们的结构显示 LHCI,尤其是 Lhca4 亚基发生了一些偏离 PSI 核心的转移,这表明从该 Lhca 亚基到 PSI 核心的能量转移存在间接联系和低效率。我们在结构中发现了五种新的脂质,其中大部分位于 Lhca 亚基和 PSI 核心之间的间隙区域。这些脂质分子可能在 Lhca 亚基与核心的结合以及超复合物的组装中发挥重要作用。因此,目前的结果为阐明这种超级复合物的光能收集、转移和组装机制提供了新的信息。
更新日期:2021-03-31
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