Construction and Application of an Escherichia coli Strain Lacking 62 Genes Responsible for the Biosynthesis of Enterobacterial Common Antigen and Flagella,Journal of Agricultural and Food Chemistry

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Construction and Application of an Escherichia coli Strain Lacking 62 Genes Responsible for the Biosynthesis of Enterobacterial Common Antigen and Flagella
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2021-03-31 , DOI: 10.1021/acs.jafc.1c00453
Jun Qiao _{1,

2} , Xin Tan _{1,

2} , Danyang Huang _{1,

2} , Hedan Li _{1,

2} , Zhen Wang _{1,

2} , Hongyu Ren _{1,

2} , Xiaoqing Hu _{1,

3} , Xiaoyuan Wang _{1,

2,

3}

Affiliation

The biosynthesis of the enterobacterial common antigen and flagella in Escherichia coli consumes lots of substrates and energy. In this study, 12 genes responsible for the biosynthesis of the enterobacterial common antigen were deleted in E. coli MG1655, resulting in WQM021. WQM021 grew better than MG1655 in both rich LB medium and minimum M9 medium. Compared with MG1655, WQM021 showed higher membrane permeability and higher production efficiency for recombinant proteins, polyhydroxyalkanoate, and l-threonine. Transcriptome analysis revealed that genes relevant to glucose consumption, glycolysis, and flagellar synthesis were significantly upregulated in WQM021. Therefore, 50 genes responsible for flagellar biosynthesis were further deleted in WQM021, resulting in WQM022. WQM022 grew better and could synthesize more polyhydroxyalkanoate and l-threonine than WQM021. The results demonstrate that the productivity of E. coli can be efficiently improved when the enterobacterial common antigen and flagella are eliminated. This strategy has guiding significance in the optimization of other industrial products and microorganisms.

中文翻译：

缺乏负责肠道细菌共同抗原和鞭毛生物合成的62个基因的大肠杆菌菌株的构建和应用

大肠杆菌中肠细菌常见抗原和鞭毛的生物合成消耗大量底物和能量。在这项研究中，大肠杆菌MG1655中删除了负责肠细菌共同抗原生物合成的12个基因，从而产生了WQM021。在丰富的LB培养基和最低M9培养基中，WQM021的生长均优于MG1655。与MG1655相比，WQM021表现出对重组蛋白，聚羟基烷酸酯，和较高的膜渗透性和提高生产效率升-苏氨酸。转录组分析显示，与葡萄糖消耗，糖酵解和鞭毛合成相关的基因在WQM021中显着上调。因此，WQM021中进一步删除了负责鞭毛生物合成的50个基因，从而产生了WQM022。与WQM021相比，WQM022生长更好，可以合成更多的聚羟基链烷酸酯和1-苏氨酸。结果表明，当消除肠细菌共同抗原和鞭毛时，可以有效地提高大肠杆菌的生产率。该策略对优化其他工业产品和微生物具有指导意义。

更新日期：2021-04-14

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