The study was based on the removal of nitrate and sulfide, and aimed to nitrite accumulation. The process of autotrophic denitrification driven by sulfide as an electron donor was investigated in a sequencing batch reactor. The research showed that autotrophic denitrification successfully started on day 22, and the removal rates of NO₃⁻-N and S^2--S were 95.8% and 100%, respectively, when the S/N molar ratio was 1.45. When the S/N ratio was reduced to 0.94, the phenomenon of NO₂⁻-N accumulation was observed. NO₂⁻-N continuously accumulated, and the maximum accumulation rate was 55.3% when the S/N ratio was 0.8. In the batch test, the study showed that NO₂⁻-N accumulation was optimal when the S/N ratio was 0.8, and the NO₂⁻-N concentration increased with increasing NO₃⁻-N concentration at the same S/N ratio. Microbial communities also changed based on the high-throughput analysis, and Proteobacteria (59.5%–84%) was the main phylum. Arenimonas (11.4%–28.2%) and uncultured_f_ Chromatiaceae (5.7%–27.5%) were the dominant bacteria, which complete denitrification and desulfurization throughout the operating system. Therefore, this study provided a theoretical basis for the simultaneous removal of NO₃⁻-N and S^2--S, as well as the accumulation of nitrite, and provided material support for anaerobic ammonia oxidation technology.

该研究基于去除硝酸盐和硫化物，并旨在亚硝酸盐的积累。在定序分批反应器中研究了由硫化物作为电子供体驱动的自养反硝化过程。研究表明，自养反硝化在第22天成功开始，当S / N摩尔比为1.45时，NO ₃ ^-- N和S ^2- -S的去除率分别为95.8％和100％。当S / N比降低至0.94时，观察到NO ₂ ^-- N蓄积的现象。NO ₂ ^-- N连续蓄积，当S / N比为0.8时，最大蓄积率为55.3％。在批量测试中，研究表明，NO ₂ ^-当S / N比为0.8时，-N累积最佳，并且在相同S / N比下，NO ₂ ^-- N浓度随着NO ₃ ^-- N浓度的增加而增加。根据高通量分析，微生物群落也发生了变化，变形杆菌（59.5％–84％）是主要菌群。支气管炎菌是沙雷菌（11.4％–28.2％）和未培养的凤尾藻科（5.7％–27.5％），它们在整个操作系统中完成了反硝化和脱硫作用。因此，本研究为同时去除NO ₃ ^-- N和S ^2-提供了理论依据。-S，以及亚硝酸盐的积累，为厌氧氨氧化技术提供了物质支持。