Ultrasensitive and Reliable Organic Field-Effect Transistor-Based Biosensors in Early Liver Cancer Diagnosis,Analytical Chemistry

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Ultrasensitive and Reliable Organic Field-Effect Transistor-Based Biosensors in Early Liver Cancer Diagnosis
Analytical Chemistry ( IF 6.7 ) Pub Date : 2021-03-29 , DOI: 10.1021/acs.analchem.1c00372
Chenfang Sun ₁ , Rui Li ₁ , Yaru Song ₁ , Xiaoqian Jiang ₁ , Congcong Zhang ₂ , Shanshan Cheng _{1,

3} , Wenping Hu _{1,

3,

4}

Affiliation

Organic field-effect transistors (OFETs) are considered as one of the cost-effective biosensor devices with rapid detection capabilities and multiparameter responses. However, the functionalization processes on normal organic devices might impact the device performance for its further sensitive and reliable sensing applications. Herein, we develop a novel organic material, 2,6-bis(4-formylphenyl)anthracene (BFPA) for use as the protective and functional layer of OFET-based biosensors, enabling ultrasensitive determination of alpha-fetoprotein (AFP) with femtomolar accuracy in human serum. By monitoring changes of the source–drain current (I_ds) and threshold voltage (V_th) electrical signals, the device exhibits improved reliability in detecting AFP biomarkers and is able to differentiate between liver cancer patients and healthy individuals. Featuring label-free determination, shorter analysis time, and lower sample volume, this ultrasensitive and reliable OFET-based biosensor displays numerous advantages over traditional strategies such as enzyme-linked immunosorbent assay and electrochemiluminescence immunoassay, demonstrating that the proposed OFET-based biosensors have broader analytical and clinical applications for early liver cancer diagnosis.

中文翻译：

基于超灵敏和可靠的基于有机场效应晶体管的生物传感器在肝癌的早期诊断中

有机场效应晶体管（OFET）被认为是具有快速检测功能和多参数响应的具有成本效益的生物传感器设备之一。但是，普通有机设备上的功能化过程可能会影响其进一步敏感和可靠的传感应用的设备性能。本文中，我们开发了一种新型有机材料2,6-双（4-甲酰基苯基）蒽（BFPA），用作基于OFET的生物传感器的保护层和功能层，从而能够以飞摩尔级精度超灵敏地测定甲胎蛋白（AFP）在人血清中。通过监测源漏电流（I _ds）和阈值电压（V _{th）的变化}）电信号，该设备在检测AFP生物标志物方面表现出更高的可靠性，并且能够区分肝癌患者和健康个体。这种超灵敏，可靠的基于OFET的生物传感器具有无标记测定，较短的分析时间和较小的样品量的特点，与传统方法（如酶联免疫吸附测定和电化学发光免疫测定）相比，具有许多优势，表明所提议的基于OFET的生物传感器具有更广泛的应用范围肝癌早期诊断的分析和临床应用。

更新日期：2021-04-20

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