Development of nanobody-based flow-through dot ELISA and lateral-flow immunoassay for rapid detection of 3-phenoxybenzoic acid,Analytical Methods

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Development of nanobody-based flow-through dot ELISA and lateral-flow immunoassay for rapid detection of 3-phenoxybenzoic acid
Analytical Methods ( IF 2.7 ) Pub Date : 2021-3-4 , DOI: 10.1039/d1ay00129a
Can Zhang ₁ , Xiaoxiao Wu , Dongyang Li , Jinnuo Hu , Debin Wan , Zhen Zhang , Bruce D Hammock

Affiliation

As a major metabolite of pyrethroid pesticides, 3-phenoxybenzoic acid (3-PBA) can be an indicator of health risk and human exposure assessment. Based on nanobodies (Nbs), we have developed a rapid flow-through dot enzyme linked immunosorbent assay (dot ELISA) and gold nanoparticle (GNP) lateral-flow immunoassay for detecting 3-PBA. The limit of detection (LOD) values for detecting 3-PBA by flow-through dot ELISA and GNP lateral-flow immunoassay were 0.01 ng mL⁻¹ and 0.1 ng mL⁻¹, respectively. The samples (urine and lake water) with and without 3-PBA were detected by both nanobody-based flow-through dot ELISA and GNP lateral-flow immunoassay, as well as liquid chromatography-mass spectrometry (LC-MS) for validation. There was good consistency between the results of the immunoassays. This demonstrated that the two developed nanobody-based immunoassays are suitable for rapid detection of 3-PBA.

中文翻译：

开发基于纳米抗体的流通点 ELISA 和横向流动免疫测定法，用于快速检测 3-苯氧基苯甲酸

3-苯氧基苯甲酸（3-PBA）作为拟除虫菊酯类农药的主要代谢产物，可作为健康风险和人体暴露评估的指标。基于纳米抗体（Nbs），我们开发了快速流通点酶联免疫吸附测定（dot ELISA）和金纳米粒子（GNP）侧流免疫测定法来检测3-PBA。通过流通点ELISA和GNP侧流免疫测定法检测3-PBA的检测限(LOD)值分别为0.01ng·mL ^-1和0.1ng·mL ^-1 。通过基于纳米抗体的流通点 ELISA 和 GNP 侧流免疫分析以及液相色谱-质谱 (LC-MS) 检测含和不含 3-PBA 的样品（尿液和湖水）进行验证。免疫测定结果之间具有良好的一致性。这表明两种开发的基于纳米抗体的免疫测定法适合快速检测3-PBA。

更新日期：2021-03-25

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