Herein, a hierarchical structure of flower-like NiCo layered double hydroxides (NiCo LDH) microspheres composed of three-dimensional (3D) ultrathin nanosheets was successfully synthesized via a facile hydrothermal approach. The formation of NiCo LDH was confirmed by various physicochemical studies, and the NiCo LDH-modified glassy carbon electrode was used as an efficient dual-functional electrocatalyst for non-enzymatic glucose and hydrogen peroxide (H₂O₂) biosensor. The host matrix of hydrotalcite NiCo LDH exhibits the enhanced electrocatalytic sensing performances with a quick response time (<3 s), wide linear range (50 nM–18.95 mM and 20 nM–11.5 mM) and lowest detection limits (S/N = 3) (10.6 and 4.4 nM) toward glucose and H₂O₂, and also it exhibits good stability, selectivity, and reproducibility. In addition, this biosensor was successfully utilized to the real-time detection of endogenous H₂O₂ produced from live cells and glucose in various biological fluids, and demonstrates that the as synthesized NiCo LDH may provide a successful pathway for physiological and clinical pathological diagnosis.

中文翻译：

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3D 花状 NiCo 层状双氢氧化物：用于活细胞中过氧化氢和生物流体中葡萄糖的非酶电化学生物传感的高效电催化剂

在此，通过简便的水热方法成功合成了由三维 (3D) 超薄纳米片组成的花状 NiCo 层状双氢氧化物 (NiCo LDH) 微球的层次结构。各种物理化学研究证实了NiCo LDH的形成，并将NiCo LDH修饰的玻碳电极用作非酶葡萄糖和过氧化氢（H ₂ O ₂）生物传感器的高效双功能电催化剂。水滑石 NiCo LDH 的主体基质表现出增强的电催化传感性能，具有快速响应时间 (<3 s)、宽线性范围 (50 nM–18.95 mM 和 20 nM–11.5 mM) 和最低检测限 (S/N = 3 ) (10.6 和 4.4 nM) 对葡萄糖和 H ₂ O ₂, 并且具有良好的稳定性、选择性和重现性。此外，该生物传感器成功地用于实时检测活细胞产生的内源性H ₂ O ₂和各种生物体液中的葡萄糖，证明合成的NiCo LDH可为生理和临床病理诊断提供一条成功的途径。 .