The Pumilio RNA-binding protein APUM24 regulates seed maturation by fine-tuning the BPM-WRI1 module in Arabidopsis,Journal of Integrative Plant Biology

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The Pumilio RNA-binding protein APUM24 regulates seed maturation by fine-tuning the BPM-WRI1 module in Arabidopsis
Journal of Integrative Plant Biology ( IF 9.3 ) Pub Date : 2021-03-17 , DOI: 10.1111/jipb.13092
Ruihua Huang ₁ , Mengling Liu ₁ , Guanping Gong ₁ , Pingzhi Wu ₂ , Barunava Patra ₃ , Ling Yuan ₃ , Hongting Qin ₁ , Xiaoxu Wang ₁ , Guohe Wang ₁ , Huimei Liao ₁ , Lu Gao ₁ , Chengwei Yang ₁ , Hongqing Li ₁ , Shengchun Zhang ₁

Affiliation

Pumilio RNA-binding proteins participate in messenger RNA (mRNA) degradation and translational repression, but their roles in plant development are largely unclear. Here, we show that Arabidopsis PUMILIO PROTEIN24 (APUM24), an atypical Pumilio-homology domain-containing protein, plays an important part in regulating seed maturation, a major stage of plant development. APUM24 is strongly expressed in maturing seeds. Reducing APUM24 expression resulted in abnormal seed maturation, wrinkled seeds, and lower seed oil contents, and APUM24 knockdown resulted in lower levels of WRINKLED 1 (WRI1), a key transcription factor controlling seed oil accumulation, and lower expression of WRI1 target genes. APUM24 reduces the mRNA stability of BTB/POZMATH (BPM) family genes, thus decreasing BPM protein levels. BPM is responsible for the 26S proteasome-mediated degradation of WRI1 and has important functions in plant growth and development. The 3′ untranslated regions of BPM family genes contain putative Pumilio response elements (PREs), which are bound by APUM24. Reduced BPM or increased WRI1 expression rescued the deficient seed maturation of apum24-2 knockdown mutants, and APUM24 overexpression resulted in increased seed size and weight. Therefore, APUM24 is crucial to seed maturation through its action as a positive regulator fine-tuning the BPM-WRI1 module, making APUM24 a promising target for breeding strategies to increase crop yields.

中文翻译：

Pumilio RNA 结合蛋白 APUM24 通过微调拟南芥中的 BPM-WRI1 模块来调节种子成熟

Pumilio RNA 结合蛋白参与信使 RNA (mRNA) 降解和翻译抑制，但它们在植物发育中的作用尚不清楚。在这里，我们展示了拟南芥PUMILIO PROTEIN24 (APUM24)，一种包含非典型 Pumilio 同源结构域的蛋白质，在调节种子成熟（植物发育的一个主要阶段）中起着重要作用。APUM24在成熟的种子中强烈表达。降低APUM24表达会导致种子成熟异常、种子起皱和种子油含量降低，APUM24敲低导致 WRINKLED 1 (WRI1) 水平降低，这是控制种子油积累的关键转录因子，以及 WRI1 靶基因的表达降低。APUM24 降低了BTB/POZMATH ( BPM ) 家族基因，从而降低 BPM 蛋白水平。BPM 负责 26S 蛋白酶体介导的 WRI1 降解，在植物生长发育中具有重要作用。BPM家族基因的 3' 非翻译区包含推定的 Pumilio 反应元件 (PRE)，它们与 APUM24 结合。降低BPM或增加WRI1表达挽救了apum24-2敲低突变体的种子成熟缺陷，APUM24过表达导致种子大小和重量增加。因此，APUM24 通过其作为微调 BPM-WRI1 模块的正调节器的作用对种子成熟至关重要，使APUM24 提高作物产量的育种策略的一个有希望的目标。

更新日期：2021-03-17

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