A novel metallo-copper phthalocyanine-based covalent-organic framework (CuTAPc-MCOF) doped with silver nanoparticles (denoted as CuTAPc-MCOF@AgNPs) was developed. CuTAPc-MCOF was synthesized using Cu(II) 4′,4′′,4′′′,4′′′′-tetra-amine phthalocyanine (CuTAPc) and 2,9-bis[p-(formyl)phenyl]-1,10-phenanthroline as building blocks through the Schiff base condensation reaction. The basic characterizations revealed that CuTAPc-MCOF demonstrated extended π-system and possessed the phenanthroline group, which can be coordinated with Ag. Consequently, it endowed the CuTAPc-MCOF@AgNPs nanohybrid with good biological compatibility, boosted electron transfer, and produced abundant catalytic active sites. As such, the CuTAPc-MCOF@AgNPs nanohybrid illustrated superior biocompatibility and excellent electrocatalytic activity, thereby showing remarkable electrocatalytic performance for electro-oxidizing nitric oxide (NO) released from living cancer cells. The sensing properties of the CuTAPc-MCOF@AgNPs-based sensor were measured by cyclic voltammetry and amperometry techniques. The CuTAPc-MCOF@AgNPs-based electrochemical sensor showed a high sensitivity of 29.1 μA μM⁻¹ cm⁻² and a low detection limit of 12.6 nM within the concentration range of 0.18 μM to 17.1 μM toward NO, together with excellent selectivity, high reproducibility and repeatability, and good stability. Overall, our sensing strategy showed potential application in detecting NO released from living cancer cells and diagnosing biological processes.

开发了一种新型的掺有银纳米粒子（表示为CuTAPc-MCOF @ AgNPs）的金属铜酞菁共价有机骨架（CuTAPc-MCOF）。CuTAPc-MCOF是使用Cu（II）4'，4''，4'''，4''''-四胺酞菁（CuTAPc）和2,9-bis [ p-（甲酰基）苯基] -1,10-菲咯啉作为席夫碱缩合反应的结构单元。基本表征表明，CuTAPc-MCOF具有扩展的π系统，并具有可与Ag配合的菲咯啉基团。因此，它赋予了CuTAPc-MCOF @ AgNPs纳米杂化物良好的生物相容性，促进了电子转移，并产生了丰富的催化活性位点。这样，CuTAPc-MCOF @ AgNPs纳米杂化物表现出优异的生物相容性和出色的电催化活性，从而显示出了对从活癌细胞中释放出的一氧化氮（NO）进行电氧化的卓越电催化性能。通过循环伏安法和安培法技术测量了基于CuTAPc-MCOF @ AgNPs的传感器的传感特性。^-1 cm ^-2且在0.18μM至17.1μM的浓度范围内对NO的检测限低至12.6 nM，同时具有出色的选择性，高重现性和可重复性以及良好的稳定性。总体而言，我们的传感策略显示了在检测从活癌细胞释放的NO和诊断生物学过程中的潜在应用。