评估柠檬酸单-，二-和三-柠檬酸根离子液体对α-胰凝乳蛋白酶的稳定性和活性的兼容性,ACS Sustainable Chemistry & Engineering

当前位置： X-MOL 学术 › ACS Sustain. Chem. Eng. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

评估柠檬酸单-，二-和三-柠檬酸根离子液体对α-胰凝乳蛋白酶的稳定性和活性的兼容性
ACS Sustainable Chemistry & Engineering ( IF 7.1 ) Pub Date : 2021-03-25 , DOI: 10.1021/acssuschemeng.1c00044
Anamika Sindhu ₁ , Meena Bisht _{1,

2} , Indra Bahadur _{2,

3} , Pannuru Venkatesu ₁

Affiliation

鉴于常规有机溶剂在全球环境和经济方面的挑战，大多数离子液体（ILs）已证明自己是“更绿色”的助溶剂，尤其是用于酶的助溶剂。然而，缺乏关于生物分子上的各种多阳离子IL系统的绿色方面的基础知识。在这方面，我们合成了柠檬酸单胆碱（[Chn] [Cit]），柠檬酸_二胆碱（[Chn] ₂ [Cit]）和柠檬酸_三胆碱（[Chn] ₃[Cit]）IL，并雇用他们来研究其对α-胰凝乳蛋白酶（α-CT）的适用性。这项研究专门研究了借助各种光谱技术提高IL阳离子比例对α-CT稳定性和活性的影响。各种光谱分析表明，随着IL的阳离子比率增加，α-CT的结构稳定性和活性得到改善。我们发现[Chn] ₃ [Cit]和[Chn] ₂ [Cit]中的α-CT相比[Chn] [Cit]具有更好的结构稳定性。有趣的是，温度依赖性荧光光谱法的结果明确阐明，与T _m相比，在[Chn] ₂ [Cit]存在下，α-CT的转变温度（T _m）提高了6°C。缓冲溶液中α-CT的含量。最终，这些结果表明，通过增加[Chn] [Cit]中的含水胆碱阳离子的摩尔比，可以增强IL稳定α-CT结构的能力。我们的结果表明，将来可能会利用多阳离子IL系统对助溶剂暴露的敏感性，作为探索经验证的稳定性研究的新生物相容性的新方法。

"点击查看英文标题和摘要"

Assessing the Compatibility of Mono-, Di-, and Tri-Cholinium Citrate Ionic Liquids for the Stability and Activity of α-Chymotrypsin

In view of the global environmental and economic challenges of conventional organic solvents, most of the ionic liquids (ILs) have proved themselves as a “greener” group of cosolvents, especially for enzymes. However, there is a lack of fundamental knowledge on the greener aspects of various multicationic IL systems on biomolecules. In this regard, we have synthesized the monocholinium citrate ([Chn][Cit]), dicholinium citrate ([Chn]₂[Cit]) and tricholinium citrate ([Chn]₃[Cit]) ILs and employed them to explore their suitability for α-chymotrypsin (α-CT). This study specifically addresses the effect of increased cationic ratio of IL on stability and activity of α-CT with the help of various spectroscopic techniques. Various spectroscopic analysis showed that there is improved structural stability and activity of α-CT as the cationic ratio of IL increase. We found better structural stability of α-CT in [Chn]₃[Cit] and [Chn]₂[Cit] as compared to that in [Chn][Cit]. Interestingly, temperature-dependent fluorescence spectroscopy results explicitly elucidate that transition temperature (T_m) of α-CT enhances by 6 °C in the presence of [Chn]₂[Cit] when compared to T_m of α-CT in buffer solution. Ultimately, these results establish that through an increase in the molar ratio of aqueous cholinium cation in [Chn][Cit] intensifies the capability of ILs to stabilize the α-CT structure. Our results suggested that multicationic IL system sensitivity to cosolvent exposure may be utlized in the future as a new approach to explore new biocompatibility toward proven stability studies.

更新日期：2021-04-05

点击分享查看原文

点击收藏

阅读更多本刊新发论文本刊介绍/投稿指南