The interest in carbon dots (CDs) as peroxidase-mimics has urged to understand the mechanism for this reaction of commercial importance. Firstly, using a theoretical-cum-experimental approach, the –COOH group was identified as an active moiety for strongly binding and degrading H₂O₂ in the presence of a reducing equivalent. Next, the stringency of different compounds containing the –COOH group was examined and identified l-glutamic acid with higher peroxidase-like activity. The –NH₂ group present in the vicinity of the –COOH group increased the activity of l-glutamic acid. Following a pyrolysis method, l-glutamic acid was transformed into CDs having a diameter of 3.18 ± 0.53 nm with its core made of pyroglutamic acid. The CD exhibited thermostability (~90 °C) and higher peroxidase-like activity than the l-glutamic acid. The higher activity of the CD was attributed to its binding affinity with ABTS and H₂O₂. The K_m and K_cat of the CD for H₂O₂ were 5.85 mM and 0.011 s⁻¹, respectively. Based on spectroscopic investigations, we confirmed the peroxidase-like activity is a surface phenomenon that did not have a significant link to the photophysical property of the CD and proposed a mechanism for the reaction. The colorimetric reaction could be reproduced on a paper platform, confirming the application potential of the CD for developing a low-cost peroxide sensor.

促使人们对过氧化物酶类似物碳点（CDs）产生兴趣，以了解这种具有商业重要性的反应的机理。首先，使用理论和实验方法，在还原当量存在的情况下，–COOH基团被确定为用于强力结合和降解H ₂ O ₂的活性部分。接下来，检查了包含–COOH基团的不同化合物的严格性，并鉴定出具有较高过氧化物酶样活性的1-谷氨酸。在–COOH基团附近存在的–NH ₂基团增加了1-谷氨酸的活性。继热解法，升用谷氨酸将谷氨酸转化为直径为3.18±0.53 nm的CD。与1-谷氨酸相比，该CD表现出热稳定性（〜90℃）和更高的过氧化物酶样活性。CD的较高活性归因于其与ABTS和H ₂ O _2的结合亲和力。CD的H ₂ O ₂的K _m和K _cat为5.85 mM和0.011 s ^-1， 分别。基于光谱学研究，我们证实了过氧化物酶样活性是一种表面现象，与CD的光物理性质没有显着联系，并提出了反应机理。比色反应可以在纸平台上进行，从而证实了CD在开发低成本过氧化物传感器方面的应用潜力。