Large bone defect repair requires biomaterials that promote angiogenesis and osteogenesis. In present work, a nanoclay (Laponite, XLS)-functionalized 3D bioglass (BG) scaffold with hypoxia mimicking property was prepared by foam replication coupled with UV photopolymerization methods. Our data revealed that the incorporation of XLS can significantly promote the mechanical property of the scaffold and the osteogenic differentiation of human adipose mesenchymal stem cells (ADSCs) compared to the properties of the neat BG scaffold. Desferoxamine, a hypoxia mimicking agent, encourages bone regeneration via activating hypoxia-inducible factor-1 alpha (HIF-1α)-mediated angiogenesis. GelMA-DFO immobilization onto BG-XLS scaffold achieved sustained DFO release and inhibited DFO degradation. Furthermore, in vitro data demonstrated increased HIF-1α and vascular endothelial growth factor (VEGF) expressions on human adipose mesenchymal stem cells (ADSCs). Moreover, BG-XLS/GelMA-DFO scaffolds also significantly promoted the osteogenic differentiation of ADSCs. Most importantly, our in vivo data indicated BG-XLS/GelMA-DFO scaffolds strongly increased bone healing in a critical-sized mouse cranial bone defect model. Therefore, we developed a novel BG-XLS/GelMA-DFO scaffold which can not only induce the expression of VEGF, but also promote osteogenic differentiation of ADSCs to promote endogenous bone regeneration.

大骨缺损修复需要促进血管生成和成骨的生物材料。在目前的工作中，通过泡沫复制结合紫外光聚合方法制备了具有缺氧模拟特性的纳米粘土（Laponite，XLS）功能化3D生物玻璃（BG）支架。我们的数据显示，与纯 BG 支架的性能相比，XLS 的掺入可以显着促进支架的机械性能和人脂肪间充质干细胞（ADSC）的成骨分化。 Desferoxamine 是一种模拟缺氧剂，通过激活缺氧诱导因子 1 α (HIF-1α) 介导的血管生成来促进骨再生。 GelMA-DFO 固定在 BG-XLS 支架上实现了 DFO 的持续释放并抑制了 DFO 降解。此外，体外数据表明，人脂肪间充质干细胞 (ADSC) 上的 HIF-1α 和血管内皮生长因子 (VEGF) 表达增加。此外，BG-XLS/GelMA-DFO支架还显着促进ADSCs的成骨分化。最重要的是，我们的体内数据表明 BG-XLS/GelMA-DFO 支架在临界尺寸的小鼠颅骨缺损模型中强烈促进骨愈合。因此，我们开发了一种新型的BG-XLS/GelMA-DFO支架，它不仅可以诱导VEGF的表达，还可以促进ADSCs的成骨分化，从而促进内源性骨再生。