This work aims to synthesize S-(4-chlorophenyl)-(pyridin-2-yl) methanol (S-CPMA) in a green, economic, and efficient way. In the water–cyclohexane liquid–liquid system, recombinant Escherichia coli (E. coli) was used as a whole-cell catalyst and retained > 60% of its catalytic activity after five reuse cycles. In situ accumulation of the substrate/product in the organic phase effectively improves substrate tolerance and reduces product inhibition and toxicity. Meanwhile, a microreaction system consisting of membrane dispersion and three-dimensional (3D) bending-microchannel was developed to successfully generate droplet swarms with an average diameter of 30 μm. Large specific surface area provided high mass transfer efficiency between phases. While the analogous reaction in a traditional stirred tank required > 270 min to achieve a yield of > 99%, in this biphasic microreaction system, the yield reached 99.6% with a high enantiomeric excess (ee) of > 99% in only 80 min. Efficient synthesis was achieved by reducing the time by 70%.

这项工作旨在以绿色，经济和有效的方式合成S-（4-氯苯基）-（吡啶-2-基）甲醇（S -CPMA）。在水环己烷液-液系统中，重组大肠杆菌（大肠杆菌）用作全细胞催化剂，并在五个重复使用循环后保留> 60％的催化活性。底物/产物在有机相中的原位累积有效地提高了底物耐受性并降低了产物抑制和毒性。同时，开发了由膜分散体和三维（3D）弯曲微通道组成的微反应系统，以成功生成平均直径为30μm的液滴群。大的比表面积提供了相之间的高传质效率。尽管在传统搅拌釜中进行类似反应需要> 270分钟才能达到> 99％的收率，但在此双相微反应系统中，仅80分钟内对映体过量（ee）≥99％的情况下收率就达到了99.6％。通过将时间减少70％，可以实现有效的合成。