当前位置：
X-MOL 学术
›
J. Agric. Food Chem.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Transcriptional Regulation of Anthocyanin Synthesis by MYB-bHLH-WDR Complexes in Kiwifruit (Actinidia chinensis)
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2021-03-22 , DOI: 10.1021/acs.jafc.0c07037
Yanfei Liu 1, 2 , Kangxun Ma 1 , Yingwei Qi 3 , Guowen Lv 1 , Xiaolin Ren 1 , Zhande Liu 1 , Fengwang Ma 1
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2021-03-22 , DOI: 10.1021/acs.jafc.0c07037
Yanfei Liu 1, 2 , Kangxun Ma 1 , Yingwei Qi 3 , Guowen Lv 1 , Xiaolin Ren 1 , Zhande Liu 1 , Fengwang Ma 1
Affiliation
|
The anthocyanin synthetic pathway is regulated centrally by an MYB-bHLH-WD40 (MBW) complex. Anthocyanin pigmentation is an important fruit quality trait in red-fleshed kiwifruit; however, the underlying regulatory mechanisms involving the MBW complex are not well understood. In this study, one R2R3MYB (AcMYBF110 expressed in fruit characteristically), one bHLH (AcbHLH1), two upstream regulators of AcbHLH1 (AcbHLH4 and AcbHLH5), and one WDR (AcWDR1) are characterized as being involved in the regulation of anthocyanin synthesis in kiwifruit. AcMYBF110 plays an important role in the regulation of anthocyanin accumulation by specifically activating the promoters of several anthocyanin pathway genes including AcCHS, AcF3′H, AcANS, AcUFGT3a, AcUFGT6b, and AcGST1. Coexpression of AcbHLH1, AcbHLH4, or AcbHLH5 together with AcMYBF110 induces much greater anthocyanin accumulation in both tobacco leaves and in Actinidia arguta fruit compared with AcMYBF110 alone. Moreover, this activation is further enhanced by adding AcWDR1. We found that both AcMYBF110 and AcWDR1 interact with all three AcbHLH factors, while AcMYBF110 also interacts with AcWDR1 to form three different MBW complexes that have different regulatory roles in anthocyanin accumulation of kiwifruit. The AcMYBF110-AcbHLH1-AcWDR1 complex directly targets the promoters of anthocyanin synthetic genes. Other features of the regulatory pathways identified include promotion of AcMYBF110, AcbHLH1,and AcWDR1 activities by this MBW complex, providing for both reinforcement and feedback regulation, whereas the AcMYBF110-AcbHLH4/5-AcWDR1 complex is indirectly involved in the regulation of anthocyanin synthesis by activating the promoters of AcbHLH1 and AcWDR1 to amplify the regulation signals of the first MBW complex.
中文翻译：
花色苷的转录调控MYB-的bHLH-WDR场馆在奇异果（猕猴桃）
花青素合成途径由MYB-bHLH-WD40（MBW）复合物集中调节。花色苷色素沉着是红肉猕猴桃的重要水果品质特征。但是，涉及MBW复合体的基本监管机制尚未得到很好的理解。在这项研究中，一种R2R3MYB（在水果中有特征性表达的AcMYBF110），一种bHLH（AcbHLH1），两个AcbHLH1上游调节剂（AcbHLH4和AcbHLH5）和一种WDR（AcWDR1）的特征在于参与猕猴桃中花色苷合成的调控。 。AcMYBF110起着通过特异性激活的几个花青素途径基因包括启动子在花色素苷的积累的调节中起重要作用ACCH上，AcF3 ' ħ，AcANS，AcUFGT3a，AcUFGT6b，和AcGST1。共表达AcbHLH1，AcbHLH4，或AcbHLH5连同AcMYBF110两个烟叶中引起更大的花青素软枣猕猴桃相比水果AcMYBF110孤单。此外，通过添加AcWDR1进一步增强了这种激活。我们发现AcMYBF110和AcWDR1都与所有三个AcbHLH因子相互作用，而AcMYBF110也与AcWDR1相互作用以形成三种不同的MBW复合物，这些复合物在猕猴桃的花青素积累中具有不同的调节作用。AcMYBF110-AcbHLH1-AcWDR1复合物直接靶向花色苷合成基因的启动子。确定的调控途径的其他特征包括通过该MBW复合物促进AcMYBF110，AcbHLH1和AcWDR1活性，同时提供增强和反馈调控，而AcMYBF110-AcbHLH4 / 5-AcWDR1复合物间接参与花青素合成的调控。激活AcbHLH1和AcWDR1的启动子 放大第一个MBW复合体的调节信号。
更新日期：2021-03-31
中文翻译：
花色苷的转录调控MYB-的bHLH-WDR场馆在奇异果（猕猴桃）
花青素合成途径由MYB-bHLH-WD40（MBW）复合物集中调节。花色苷色素沉着是红肉猕猴桃的重要水果品质特征。但是，涉及MBW复合体的基本监管机制尚未得到很好的理解。在这项研究中，一种R2R3MYB（在水果中有特征性表达的AcMYBF110），一种bHLH（AcbHLH1），两个AcbHLH1上游调节剂（AcbHLH4和AcbHLH5）和一种WDR（AcWDR1）的特征在于参与猕猴桃中花色苷合成的调控。 。AcMYBF110起着通过特异性激活的几个花青素途径基因包括启动子在花色素苷的积累的调节中起重要作用ACCH上，AcF3 ' ħ，AcANS，AcUFGT3a，AcUFGT6b，和AcGST1。共表达AcbHLH1，AcbHLH4，或AcbHLH5连同AcMYBF110两个烟叶中引起更大的花青素软枣猕猴桃相比水果AcMYBF110孤单。此外，通过添加AcWDR1进一步增强了这种激活。我们发现AcMYBF110和AcWDR1都与所有三个AcbHLH因子相互作用，而AcMYBF110也与AcWDR1相互作用以形成三种不同的MBW复合物，这些复合物在猕猴桃的花青素积累中具有不同的调节作用。AcMYBF110-AcbHLH1-AcWDR1复合物直接靶向花色苷合成基因的启动子。确定的调控途径的其他特征包括通过该MBW复合物促进AcMYBF110，AcbHLH1和AcWDR1活性，同时提供增强和反馈调控，而AcMYBF110-AcbHLH4 / 5-AcWDR1复合物间接参与花青素合成的调控。激活AcbHLH1和AcWDR1的启动子 放大第一个MBW复合体的调节信号。
京公网安备 11010802027423号