Microchimica Acta ( IF 5.3 ) Pub Date : 2021-03-19 , DOI: 10.1007/s00604-021-04785-2 Huimin Wan 1 , Yufei Wang 1 , Juan Chen 1 , Hong-Min Meng 1 , Zhaohui Li 1, 2
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A two-dimensional (2D) Co-MOF nanosheet-based nanozyme was developed for colorimetric detection of disease-related biomolecules. The prepared 2D Co-MOFs exhibited ultrahigh peroxidase catalytic activity. 2D Co-MOFs can catalyze the oxidation of colorless 3,3′,5,5′-tetramethylbenzidine (TMB) to the blue product oxTMB, accompanying an obvious change of absorption value at 652 nm. However, alkaline phosphatase can catalyze the hydrolysis of L-ascorbic acid-2-phosphate to produce ascorbic acid which can reduce the oxTMB to TMB, resulting in an obvious color fading. Therefore, by recording the change of absorption value at 652 nm, the 2D Co-MOF nanosheets were used to detect ascorbic acid (AA) and alkaline phosphatase (ALP). The limit of detection for AA and ALP was 0.47 μM and 0.33 U L−1, respectively. The limit of quantification for AA and ALP was 1.56 μM and 1.1 U L−1, respectively. The developed nanozyme was successfully used to determine alkaline phosphatase in clinical human serum samples and the results were consistent with those provided by the hospital. Furthermore, by integrating 2D Co-MOF nanosheets with image recognition and data processing function fixed on a smartphone, a portable test of ascorbic acid was reached.
Graphical abstract
中文翻译：
具有超高过氧化物酶催化活性的二维 Co-MOF 纳米片基纳米酶用于检测人血清样品中的生物分子
开发了一种基于二维 (2D) Co-MOF 纳米片的纳米酶，用于比色检测疾病相关的生物分子。制备的二维 Co-MOFs 表现出超高的过氧化物酶催化活性。2D Co-MOFs可以催化无色3,3',5,5'-四甲基联苯胺（TMB）氧化为蓝色产物oxTMB，同时在652 nm处吸收值发生明显变化。但碱性磷酸酶可催化L-抗坏血酸-2-磷酸水解生成抗坏血酸，将oxTMB还原为TMB，导致明显的褪色。因此，通过记录 652 nm 处吸收值的变化，二维 Co-MOF 纳米片可用于检测抗坏血酸 (AA) 和碱性磷酸酶 (ALP)。AA 和 ALP 的检测限为 0.47 μM 和 0.33 U L -1， 分别。AA 和 ALP 的定量限分别为 1.56 μM 和 1.1 U L -1。开发的纳米酶成功用于临床人血清样品中碱性磷酸酶的测定，结果与医院提供的结果一致。此外，通过将二维 Co-MOF 纳米片与固定在智能手机上的图像识别和数据处理功能相结合，实现了抗坏血酸的便携式测试。
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