Multicolor Two‐Photon Nanosystem for Multiplexed Intracellular Imaging and Targeted Cancer Therapy,Angewandte Chemie International Edition

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Multicolor Two‐Photon Nanosystem for Multiplexed Intracellular Imaging and Targeted Cancer Therapy
Angewandte Chemie International Edition ( IF 16.1 ) Pub Date : 2021-03-19 , DOI: 10.1002/anie.202103027
Yong-Xiang Wu _{1,

2} , Dailiang Zhang ₁ , Xiaoxiao Hu ₁ , Ruizi Peng _{1,

3} , Junbin Li ₁ , Xiaobing Zhang ₁ , Weihong Tan _{1,

3,

4}

Affiliation

The novel theranostic nanosystems based on two‐photon fluorescence can achieve higher spatial resolution of deep tissue imaging for simultaneous diagnosis and therapy of a variety of cancers. Herein, we have designed and prepared FRET‐based two‐photon mesoporous silica nanoparticles (MTP–MSNs) for single‐excitation multiplexed intracellular imaging and targeted cancer therapy for the first time. This nanosystem includes two constituents, containing (1) multicolor two‐photon mesoporous silica nanoparticles and (2) cancer cell‐targeting aptamers that act as gatekeepers for MTP–MSNs. After incubation with cancer cells, the Dox‐loaded and aptamer‐capped MTP–MSNs could be internalized into the cells, opening the pores and releasing the drug. Furthermore, using two‐photon multicolor fluorescence, MTP–MSNs could serve as good contrast agents for multicolor two‐photon intracellular imaging with increased imaging depth and improved spatial localization of tissue. In sum, these multicolor MTP–MSNs provide a promising system for traceable targeted cancer therapy with further applications in multiplex intracellular imaging and the screening of drug.

中文翻译：

用于多重细胞内成像和靶向癌症治疗的多色双光子纳米系统

基于双光子荧光的新型治疗诊断纳米系统可以实现更高的深部组织成像空间分辨率，用于同时诊断和治疗多种癌症。在此，我们首次设计并制备了基于 FRET 的双光子介孔二氧化硅纳米粒子 (MTP-MSNs)，用于单激发多路细胞内成像和靶向癌症治疗。该纳米系统包括两种成分，包含（1）多色双光子介孔二氧化硅纳米粒子和（2）作为 MTP-MSN 看门人的靶向癌细胞的适体。与癌细胞孵育后，加载了 Dox 和适配体的 MTP-MSNs 可以被内化到细胞中，打开毛孔并释放药物。此外，利用双光子多色荧光，MTP-MSNs 可以作为多色双光子细胞内成像的良好造影剂，具有增加的成像深度和改善的组织空间定位。总之，这些多色 MTP-MSN 为可追踪的靶向癌症治疗提供了一个有前景的系统，并进一步应用于多重细胞内成像和药物筛选。

更新日期：2021-05-17

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