Bacterial quorum sensing (QS) molecules are involved in the coordination of certain behaviors such as biofilm formation, virulence and antibiotic resistance. QS molecules (autoinducers) and their corresponding receptors have been recognized as important therapeutic targets for drug-resistant infections and biofilm-associated infections (BAI). This study assessed the multiple biological effects of homogentisic acid γ-lactone (HgAL), a furanone derivative. The anti-QS and anti-biofilm effects of HgAL against PAO1 strain were evaluated using CLSM, SEM, HPLC and other biochemical methods The results showed that HgAL could effectively inhibit the production of pyocyanin and extracellular matrix, as well as reduce the adherence ability and biofilm formation of Pseudomonas aeruginosa. Inhibition of virulence is attributed to the suppressive effect of HgAL on biosynthesis of 3-oxo-C12-HSL and C4-HSL (two kinds of QS signaling molecules in P. aeruginosa). Our results support HgAL as a potential agent for prevention of BAI in the healthcare settings.

中文翻译：

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高纯酸γ-内酯通过猝灭群体感应信号分子抑制铜绿假单胞菌的毒力因子

细菌群体感应（QS）分子参与某些行为的协调，例如生物膜形成，毒力和抗生素抗性。QS分子（自诱导剂）及其相应的受体已被认为是耐药性感染和生物膜相关感染（BAI）的重要治疗靶标。这项研究评估了呋喃酮衍生物高龙胆酸γ-内酯（HgAL）的多种生物学作用。用CLSM，SEM，HPLC和其他生化方法评价了HgAL对PAO1菌株的抗QS和抗生物膜作用。结果表明，HgAL可以有效地抑制绿脓蛋白和细胞外基质的产生，并降低其粘附能力。铜绿假单胞菌的生物膜形成。毒力的抑制归因于HgAL对3-oxo-C12-HSL和C4-HSL（铜绿假单胞菌中的两种QS信号分子）生物合成的抑制作用。我们的结果支持HgAL作为在医疗机构中预防BAI的潜在药物。