Abstract A ratiometric fluorescent hybrid nanoprobe CDs-1 for arginine (Arg), exhibiting high sensitivity (the limit of detection, LOD, being 6.5 × 10−8 mol/L) and excellent selectivity and anti-interference ability, was fabricated through fluorescence resonance energy transfer (FRET) and the electrostatic attraction between positively-charged hemicyanine molecules and negatively-charged carbon dots (CDs). Arg can be quantitatively detected in the concentration range from 6.0 × 10−5 mol/L to 2.7 × 10−4 mol/L. Further, due to its ability to target mitochondrion and low cytotoxicity, intracellular Arg was successfully tracked through ratiometric fluorescence imaging.

中文翻译：

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基于FRET的混合纳米探针基于荧光定量检测线粒体中的精氨酸

摘要通过荧光共振反应制备了精氨酸（Arg）的比例荧光杂交纳米探针CDs-1，具有很高的灵敏度（检测限，LOD，为6.5×10-8 mol / L），选择性和抗干扰性极佳。能量转移（FRET）和带正电的半菁分子与带负电的碳点（CD）之间的静电吸引。可以在6.0×10-5 mol / L至2.7×10-4 mol / L的浓度范围内定量检测Arg。此外，由于其靶向线粒体的能力和低细胞毒性，因此通过比例荧光成像成功地追踪了细胞内的Arg。