Background: Rheumatoid arthritis (RA) is an autoimmune disease responsible for maximum human morbidity in modern life, whereas oxidative stress is the ultimate potential biomarker for determining disease activity in patients with RA.

Objective: The present study scientifically validated the effectiveness of antioxidants commonly present in different food supplements to neutralize the free radicals mediated oxidative stress in isolated peripheral blood mononuclear lymphocytes (PBML) of patients with RA.

Methods: The study population included patients with Rheumatoid arthritis, RA (n =15), who fulfilled the American College of Rheumatology criteria for RA. Peripheral blood was collected, and isolated mononuclear lymphocyte cells (PBML) were pretreated with phorbol myristate acetate (PMS) and furthermore, incubated with different concentrations of Naringenin, β carotene and Nacetyl cysteine (NAC) in an ex vivo condition. The resultant cell lysate was used for further studies for the determination of other oxidative biomarkers. The increase of superoxide and nitric oxide production was observed when PBML was treated PMS.

Results: Importantly, the increased oxidative stress was effectively decreased by the selected plantderived compounds β-carotene and naringenin.

Conclusion: The study scientifically evaluated the efficacy of the molecules validated by one-way ANOVA, followed by Dunnett’s post hoc test of significance. Collectively, our results indicate that both β carotene and naringenin may be a promising non-toxic food supplement in attenuating the oxidative stress associated pathology in RA, meriting further pharmacological studies on other inflammatory cells like neutrophils.

背景：类风湿关节炎（RA）是一种自身免疫性疾病，可导致现代人类最大的发病率，而氧化应激是确定RA患者疾病活动的最终潜在生物标志物。

目的：本研究科学验证了各种食品补充剂中常见的抗氧化剂对中和RA患者离体外周血单个核淋巴细胞（PBML）自由基介导的氧化应激的作用。

方法：研究人群包括类风湿关节炎（RA）（n = 15），符合美国风湿病学会（RA）标准。收集外周血，并用佛波肉豆蔻酸酯乙酸盐（PMS）预处理分离的单核淋巴细胞（PBML），然后在离体条件下与不同浓度的柚皮素，β胡萝卜素和N乙酰基半胱氨酸（NAC）一起孵育。所得的细胞裂解物用于进一步研究以确定其他氧化生物标志物。当PBML处理为PMS时，观察到超氧化物和一氧化氮产生的增加。

结果：重要的是，选定的植物衍生化合物β-胡萝卜素和柚皮素有效降低了氧化应激的增加。

结论：该研究科学评估了通过单因素方差分析验证的分子的功效，然后进行了邓内特的事后意义检验。总体而言，我们的研究结果表明，β胡萝卜素和柚皮素都可能是有希望的无毒食品补充剂，可减轻RA中与氧化应激相关的病理，从而需要对其他炎性细胞（如中性粒细胞）进行进一步的药理研究。