By silica gel, MCI column chromatographic and preparative HPLC (high performance liquid chromatography) technologies, six nitrogen compounds were isolated from the whole parts of Gymnotheca involucrata. By using spectroscopic techniques including NMR and MS, these compounds were identified as 2-(1-hydroxy-6-methoxy-4-oxocyclohexa-2-ene- 1-yl)acetonitrile (1), 10-methoxy-1-oxa-3-azaspiro(4.5)dec-2,6-diene-8-one (2), 2-(1-hydroxy-4-oxocyclohexa-2,5-dien-1-yl)- acetonitrile (3), 1-oxa-3-azaspiro(4.5)dec-2-ene-8-one (4), 1-oxa-3-azaspiro(4.5)dec-2,6-diene-8-one (5) and 4-hydroxybenzyl acetonitrile (6). Compounds 1 and 2 are new compounds, compound 3 is a new natural product, and compounds 4~6 were isolated from this plant for the first time. Keywords Gymnotheca involucrata; saururaceae; nitrogen compound; chemical constituent

中文翻译：

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Gymnotheca involucrata 中的两种新氮化合物

通过硅胶、MCI柱色谱和制备型HPLC（高效液相色谱）技术，从裸露藻全部分中分离出6种含氮化合物。通过使用包括 NMR 和 MS 在内的光谱技术，这些化合物被鉴定为 2-(1-羟基-6-甲氧基-4-氧代环己-2-烯-1-基)乙腈 (1)、10-甲氧基-1-氧杂- 3-azaspiro(4.5)dec-2,6-diene-8-one (2), 2-(1-hydroxy-4-oxocyclohexa-2,5-dien-1-yl)-乙腈 (3), 1- oxa-3-azaspiro(4.5)dec-2-ene-8-one (4), 1-oxa-3-azaspiro(4.5)dec-2,6-diene-8-one (5) 和 4-hydroxybenzyl 乙腈(6). 化合物1、2为新化合物，化合物3为天然新产物，化合物4~6为首次从该植物中分离得到。关键词 Gymnotheca involucrata; 三色堇科；氮化合物；化学成分