This work evaluates the performance of different biochar-amended anaerobic digestion systems. The Fourier Transform Infrared analysis showed that more ordered aromatic groups formed and the aromatization degree increased with the rise of gasification temperature. The biochar produced at 900 °C still showed an excellent ability to maintain the stability of anaerobic digestion performance, where the specific methane yield content steadily reached 742 mL CH₄/g ethanol. Besides, the enzymatic activity test indicated an improved performance with the addition of biochar obtained at gasification temperature. The relationship between the microbial community and metabolism pathways result are signified due to the direct interspecies electron transfer among Pseudomonas or Candidatus cloacimonas and Methanosaeta via biochar. These links have promoted the methane metabolism pathway of acetate decarboxylation. Therefore, the current study helps better understand the influence of surface functional groups of biochar at different temperatures on anaerobic digestion performance.

这项工作评估了不同生物炭改良的厌氧消化系统的性能。傅立叶变换红外分析表明，随着气化温度的升高，形成的芳族基团数量更多，芳构化程度增加。在900°C下产生的生物炭仍具有出色的维持厌氧消化性能稳定性的能力，其中特定的甲烷产率稳定地达到742 mL CH ₄ / g乙醇。此外，酶活性测试表明通过添加在气化温度下获得的生物炭可以改善性能。微生物群落与代谢途径结果之间的关系是由于假单胞菌或假单胞菌之间直接的种间电子转移而引起的。通过生物炭进行的假丝酵母念珠菌和甲烷菌。这些联系促进了乙酸脱羧的甲烷代谢途径。因此，本研究有助于更好地了解不同温度下生物炭的表面官能团对厌氧消化性能的影响。