Thick-Shell CdSe/ZnS/CdZnS/ZnS Core/Shell Quantum Dots for Quantitative Immunoassays,ACS Applied Nano Materials

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Thick-Shell CdSe/ZnS/CdZnS/ZnS Core/Shell Quantum Dots for Quantitative Immunoassays
ACS Applied Nano Materials ( IF 5.3 ) Pub Date : 2021-03-10 , DOI: 10.1021/acsanm.0c03483
Yanbing Lv ₁ , Yucheng Yuan ₂ , Ning Hu ₁ , Na Jin ₂ , Dangdang Xu ₁ , Ruili Wu ₁ , Huaibin Shen ₁ , Ou Chen ₂ , Lin Song Li ₁

Affiliation

Colloidal quantum dots (QDs) have been broadly applied as fluorescent labeling materials in the fields of bio-imaging, sensing, and detection, in which the optical performances and stability of the utilized QDs are vital. Despite significant efforts in developing such materials, it is still challenging to fabricate QDs with high photoluminescence quantum yields (PL QYs) while concurrently possessing high particle and optical stabilities, limiting the further development of QD-based biological labeling and detection techniques. Herein, we report a synthesis of giant CdSe/ZnS/CdZnS/ZnS core/shell QDs with an ultrathick shell (∼36 monolayers) and high PL QYs (>80%). We further employ the as-synthesized QDs for C-reactive protein detection, which reach nearly a 3-fold enhancement in detection sensitivity (∼0.41 ng/mL) and a 2-fold increase in shelf life, in comparison to the traditional thick-shell QDs (i.e., CdSe/CdS/ZnS core/shell QDs) or commercially available core/shell QDs. We postulate that the performance improvements in protein detection are due to the use of the developed core/shell QDs with high particle stability and PL QYs and large surface area (facilitating surface antibody coupling). Our study demonstrates the viability of applying high-quality core/shell QDs for protein detection with high sensitivity and accuracy.

中文翻译：

用于定量免疫测定的厚壳CdSe / ZnS / CdZnS / ZnS核/壳量子点

胶体量子点（QD）已被广泛用作生物成像，传感和检测领域中的荧光标记材料，在这些领域中，所用QD的光学性能和稳定性至关重要。尽管在开发此类材料上付出了巨大的努力，但制造具有高光致发光量子产率（PL QYs）的QD仍然具有挑战性，同时又具有高的粒子和光学稳定性，从而限制了基于QD的生物标记和检测技术的进一步发展。本文中，我们报道了具有超厚壳层（〜36个单层）和高PL QYs（> 80％）的巨型CdSe / ZnS / CdZnS / ZnS核/壳量子点的合成。我们进一步将合成的QD用于C反应蛋白检测，检测灵敏度几乎提高了3倍（〜0。例如CdSe / CdS / ZnS核/壳QD）或市售的核/壳QD。我们推测蛋白质检测性能的提高是由于使用了具有高颗粒稳定性，PL QY和大表面积（促进表面抗体偶联）的已开发核/壳QD。我们的研究证明了应用高质量的核/壳QD进行蛋白质检测的可行性，具有很高的灵敏度和准确性。

更新日期：2021-03-26

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