The influence of copper ion (Cu²⁺) on anaerobic ammonium oxidation (anammox) performance and microbial community structures after oxytetracycline (OTC) stress recovery were assessed. Experimental results demonstrated that anammox performance were stressed by 1.0 mg L⁻¹ Cu²⁺ and inhibitions were reversible with total nitrogen removal rate higher than 3.08 ± 0.2 kg N m⁻³ d⁻¹. The residual OTC in the anammox sludge could combine with Cu²⁺ introduced and thereby retarded inhibition on performance in the presence of 2.0 mg L⁻¹ Cu²⁺. Moreover, the positive relation of dominant bacterium Ca. Anammoxoglobus with the abundance of functional genes and parts of antibiotic resistance genes were observed, suggesting that regain of performance was the results of the gradual domestication of latent resistant species after inhibition. This investigation reveals new insights into resistance of anammox performance for Cu²⁺ and OTC.

评估了土霉素（OTC）胁迫恢复后铜离子（Cu ²⁺）对厌氧铵氧化（anammox）性能和微生物群落结构的影响。实验结果表明，1.0 mg L ^-1 Cu ²⁺可以增强厌氧氨氧化性能，且总氮去除率高于3.08±0.2 kg N m ^-3 d ^-1时，抑制作用是可逆的。厌氧氨氧化污泥中残留的OTC可能与引入的Cu ²⁺结合，从而在2.0 mg L ^-1 Cu ²⁺的存在下延迟了对性能的抑制。此外，优势细菌钙的正相关。观察到具有大量功能基因和部分抗生素抗性基因的厌氧小球，这表明性能的恢复是抑制后潜伏的抗性物种逐渐驯化的结果。这项调查揭示了对Cu ²⁺和OTC的厌氧氨氧化性能的抵抗力的新见解。