体外纤维蛋白原纤维组装原理,Macromolecular Bioscience

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体外纤维蛋白原纤维组装原理
Macromolecular Bioscience ( IF 4.4 ) Pub Date : 2021-03-09 , DOI: 10.1002/mabi.202000412
Stephani Stamboroski _{1,

2} , Arundhati Joshi ₂ , Paul-Ludwig Michael Noeske _{1,

3} , Susan Köppen _{4,

5} , Dorothea Brüggemann _{2,

5}

Affiliation

纤维蛋白原纳米纤维具有模拟天然血凝块结构的能力，因此在伤口愈合和个性化再生医学中具有巨大的应用潜力。尽管存在多种策略来在体外诱导纤维蛋白原的原纤维形成，但对潜在机制和相关的长度尺度知之甚少。因此，在这份手稿中，回顾了体外纤维蛋白原原纤维发生的研究现状。首次将导致纤维蛋白原分子组装成纤维的多种因素分为三个主要组：底物相互作用、变性和非变性缓冲条件。根据综述中的荟萃分析，得出的结论是，纤维蛋白原的组装是由跨越不同长度尺度的几种机制驱动的。在这些过程中，与基底材料的表面化学相比，某些缓冲条件，特别是盐的存在，在纤维蛋白原自组装过程中起主要作用。然而，要为未来的组织工程应用定制具有明确结构-功能关系的纤维蛋白原支架，仍然需要了解这些因素在纤维组装过程中的具体作用。因此，建议未来将实验和模拟研究相结合，以了解纤维蛋白原的分子间相互作用，从而诱导可溶性纤维蛋白原组装成固体纤维。为了为未来的组织工程应用定制具有确定的结构-功能-关系的纤维蛋白原支架，仍然需要了解这些因素在纤维组装过程中的具体作用。因此，建议未来将实验和模拟研究相结合，以了解纤维蛋白原的分子间相互作用，从而诱导可溶性纤维蛋白原组装成固体纤维。为了为未来的组织工程应用定制具有确定的结构-功能-关系的纤维蛋白原支架，仍然需要了解这些因素在纤维组装过程中的具体作用。因此，建议未来将实验和模拟研究相结合，以了解纤维蛋白原的分子间相互作用，从而诱导可溶性纤维蛋白原组装成固体纤维。

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Principles of Fibrinogen Fiber Assembly In Vitro

Fibrinogen nanofibers hold great potential for applications in wound healing and personalized regenerative medicine due to their ability to mimic the native blood clot architecture. Although versatile strategies exist to induce fibrillogenesis of fibrinogen in vitro, little is known about the underlying mechanisms and the associated length scales. Therefore, in this manuscript the current state of research on fibrinogen fibrillogenesis in vitro is reviewed. For the first time, the manifold factors leading to the assembly of fibrinogen molecules into fibers are categorized considering three main groups: substrate interactions, denaturing and non‐denaturing buffer conditions. Based on the meta‐analysis in the review it is concluded that the assembly of fibrinogen is driven by several mechanisms across different length scales. In these processes, certain buffer conditions, in particular the presence of salts, play a predominant role during fibrinogen self‐assembly compared to the surface chemistry of the substrate material. Yet, to tailor fibrous fibrinogen scaffolds with defined structure–function‐relationships for future tissue engineering applications, it still needs to be understood which particular role each of these factors plays during fiber assembly. Therefore, the future combination of experimental and simulation studies is proposed to understand the intermolecular interactions of fibrinogen, which induce the assembly of soluble fibrinogen into solid fibers.

更新日期：2021-05-14

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