鉴定山药蛋白<em>体外</em>胃肠道消化释放的生物活性肽（<em>Dioscorea cayennensis</em>）。,Food Research International

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鉴定山药蛋白体外胃肠道消化释放的生物活性肽（Dioscorea cayennensis）。
Food Research International ( IF 7.0 ) Pub Date : 2021-03-09 , DOI: 10.1016/j.foodres.2021.110286
Edilza Silva do Nascimento ₁ , Katya Anaya ₂ , Julia Mariano Caju de Oliveira ₃ , José Thalles Jocelino Gomes de Lacerda ₄ , Michael Edward Miller ₅ , Meriellen Dias ₆ , Maria Anita Mendes ₆ , Juliana de Azevedo Lima Pallone ₇ , Clarice Weis Arns ₅ , Maria Aparecida Juliano ₄ , Tatiane Santi Gadelha ₈ , Maria Teresa Bertoldo Pacheco ₉ , Carlos Alberto de Almeida Gadelha ₁

Affiliation

Department of Food Engineering, Post-Graduate Program in Food Science and Technology, Technology Center, Federal University of Paraiba, João Pessoa, PB, Brazil; Department of Molecular Biology, Laboratory of Structural Proteomics, Federal University of Paraíba, João Pessoa, PB, Brazil.
Faculty of Health Sciences of Trari, Federal University of Rio Grande do Norte, Santa Cruz, RN, Brazil.
Department of Molecular Biology, Laboratory of Structural Proteomics, Federal University of Paraíba, João Pessoa, PB, Brazil.
Department of Biophysics, Federal University of São Paulo, São Paulo, SP, Brazil.
Department of Genetics, Evolution and Bioagents, Institute of Biology, State University of Campinas, Campinas, SP, Brazil.
Department of Chemical Engineering, University of São Paulo, São Paulo, SP, Brazil.
Department of Food Science, School of Food Engineering, State University of Campinas, Campinas, SP, Brazil.
Department of Molecular Biology, Laboratory of Genetic Biochemistry and Radiology, Federal University of Paraíba, João Pessoa, PB, Brazil.
Institute of Food Technology, Center of Food Science and Quality, Campinas, SP, Brazil.

由于生物活性肽对人类健康的贡献，已经对其进行了广泛的研究。这项研究旨在鉴定由山药蛋白质的体外胃肠道消化产生的生物活性肽。将山药浓缩蛋白（YPC）进行模拟消化。胃水解物（GPH）和总胃肠道水解物（GIPH）具有通过nanoLC-ESI-MS / MS鉴定的肽。对肽序列进行数据库驱动（BIOPEP）生物活性搜索。体外测试包括：抗氧化活性，DNA损伤保护，ACE抑制活性和对大肠杆菌，沙门氏菌（Salmonella sp。）的抗菌活性。和单核细胞增生李斯特菌。模拟消化产生了小肽段（大多数是MW <3500 Da），其中一些具有在计算机上预测的潜在生物活性序列。尽管GIPH表现出更强的DNA损伤保护和对大肠杆菌的抗菌活性，但在GPH和GIPH中都检测到了生物学活性。纱线蛋白的消化释放出有前途的生物活性肽，这些肽可以有助于预防细菌感染和慢性退行性疾病，对人类健康具有有益作用。

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更新日期：2021-03-21

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