In this study, synthesized N'-(2-methoxybenzylidene)-4-hydroxybenzohydrazide and N'-(4-nitrobenzylidene)-4-hydroxybenzohydrazide in two step reaction by using methylparaben as starting material has been performed. Methylparaben was treated with hydrazine hydrate to obtain 4-hydroxybenzohydrazide. The reaction was carried out by microwave irradiation resulting 91 % yield. The obtained compound was then reacted with 2-methoxbenzaldehyde or 4nitrobenzaldehyde to accomplish the target molecule, N'-(2-methoxybenzylidene)-4-hydroxybenzohydrazide and N'-(4nitrobenzylidene)-4-hydroxybenzohydrazide in 55% and 72% yield respectively. Identification of N'-(2methoxybenzylidene)-4-hydroxy benzo hydrazide and N'-(4-nitrobenzylidene)-4-hydroxybenzohidroksida was performed by FT-IR, MS, 1H-NMR, and 13 C-NMR spectroscopy. In silico study was done with receptor pdb 1C14. The N'-(4nitrobenzylidene)-4-hydroxybenzohydrazide exhibited antimicrobial activity against Escherichia coli (MIC=31.3ppm), Bacillus subtilis (MIC=500 ppm). Antimicrobial activity of N'-(2-methoxybenzylidene)-4-hydroxybenzohydrazide against Bacillus subtilis (MIC=31.3ppm) and MIC=500ppm against Escherichiacoli.

中文翻译：

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N'-（2-甲氧基亚苄基）-4-羟基苯甲酰肼和N'-（4-硝基亚苄基）-4-羟基苯甲酰肼的合成及抗菌活性

在该研究中，已经以对羟基苯甲酸甲酯为原料，在两步反应中合成了N'-（2-甲氧基亚苄基）-4-羟基苯并肼和N'-（4-硝基亚苄基）-4-羟基苯并肼。用水合肼处理对羟基苯甲酸甲酯，得到4-羟基苯并酰肼。通过微波辐射进行反应，得到91％的收率。然后将获得的化合物与2-甲氧基苯甲醛或4-硝基苯甲醛反应以分别以55％和72％的收率完成目标分子N'-（2-甲氧基亚苄基）-4-羟基苯并肼和N'-（4-硝基亚苄基）-4-羟基苯并肼。N'-（2-甲氧基亚苄基）-4-羟基苯甲酰肼和N'-（4-硝基亚苄基）-4-羟基苯甲酰肼的鉴定是通过FT-IR，MS，1H-NMR和13 C-NMR光谱进行的。在计算机上使用受体pdb 1C14进行研究。N'-（4-硝基亚苄基）-4-羟基苯甲酰肼表现出对大肠杆菌（MIC = 31.3ppm），枯草芽孢杆菌（MIC = 500ppm）的抗菌活性。N'-（2-甲氧基亚苄基）-4-羟基苯甲酰肼对枯草芽孢杆菌（MIC = 31.3ppm）和MIC = 500ppm对大肠杆菌的抗菌活性。