Chitin has become a desirable raw material used in various areas of life. The black soldier fly (Hermetia illucens) can be a source of this substance. In the literature, there are many methods of obtaining chitin but there is no one universal method of isolating it. In this publication, we present various procedures for the isolation of chitin from H. illucens pupal exuviae. The obtained chitin variants were characterized using different techniques (optical and confocal microscopy, FTIR, XRD, EDX, thermogravimetric analysis). The tested chitin isolated with an efficiency of 5.69–7.95% was the α form with a crystallinity degree of 60% and maximum degradation temperature of 392 °C. Furthermore, we characterized the nickel ion biosorption process on chitin and proposed the mechanism of this process to be ion exchange and complexation. There have been no such studies thus far on the isolation of chitin from H. illucens exuviae or on the biosorption of nickel ions on this type of biosorbent. The conducted research can be used to develop the application of chitin as a metal biosorbent that can be obtained with relatively high efficiency and good sorption properties.

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黑水虻中几丁质的分离及其在金属吸附中的应用

甲壳素已成为应用于生活各个领域的理想原料。黑水虻（Hermetia illucens）可能是这种物质的来源。在文献中，有许多获得甲壳素的方法，但没有一种通用的分离方法。在本出版物中，我们介绍了从H. illucens蛹蜕皮中分离几丁质的各种程序。使用不同的技术（光学和共焦显微镜、FTIR、XRD、EDX、热重分析）对获得的几丁质变体进行了表征。测试的甲壳素分离效率为5.69%~7.95%，为α晶型，结晶度为60%，最高降解温度为392℃。此外，我们还表征了甲壳素上镍离子的生物吸附过程，并提出该过程的机理是离子交换和络合。迄今为止，还没有关于从H. illucens蜕皮中分离几丁质或镍离子在此类生物吸附剂上的生物吸附的研究。所进行的研究可用于开发甲壳素作为金属生物吸附剂的应用，其具有相对较高的效率和良好的吸附性能。