Entomopathogenic fungi have been used as important biological control agents throughout the world. To improve the biocontrol efficacy of entomopathogenic fungi, many genes have been used to improve fungal virulence or tolerance to adverse conditions via modulating their expression with strong promoters. The Magas1 gene is specifically expressed during appressorium formation and contributes to the virulence in Metarhizium acridum. In this study, we analyzed the functional region of the promoter of Magas1 gene (PMagas1) in M. acridum using 5'-deletion technique with enhanced green fluoresces protein (EGFP) as a reporter. Results showed the full length of the PMagas1 was at least 897 bp. Two regions (-897 to -611 bp and -392 to -328 bp) were essential for the activity of PMagas1. An engineered M. acridum strain was constructed with PMagas1 driving the expression of a subtilisin-like proteinase gene Pr1A (PMagas1-PR1A). Bioassay showed that the virulence was significantly increased in PMagas1-PR1A strain compared to wild type strain. Pmagas1 promoter is suitable for the overexpression of some genes during the infection of entomopathogenic fungi, which avoids the waste of nutritional resources and the influence on other fungal characteristics during the saprophytic process of constitutive promoter.

昆虫病原真菌在世界范围内已被用作重要的生物防治剂。为了提高昆虫病原真菌的生物防治功效，许多基因已被用于通过用强启动子调节它们的表达来提高真菌毒力或对不利条件的耐受性。所述Magas1基因期间附着胞的形成并有助于在毒力中特异性表达绿僵acridum。在这项研究中，我们使用增强型绿色荧光蛋白 (EGFP) 的 5'-缺失技术作为报告基因分析了M. acridum中Magas1基因 ( PMagas1 )启动子的功能区域。结果显示PMgas1 的全长至少为 897 bp。两个区域（-897 到 -611 bp 和 -392 到 -328 bp）对于PMagas1的活性是必不可少的。经工程改造的M. acridum菌株用构建PMagas1驱动枯草杆菌蛋白酶类蛋白酶基因的表达的PR1a（PMagas1-的PR1a）。生物测定表明，与野生型菌株相比，PMagas1-PR1A菌株的毒力显着增加。Pmagas1启动子适用于昆虫病原真菌侵染过程中部分基因的过表达，避免了组成型启动子腐生过程中营养资源的浪费和对其他真菌特性的影响。