Objective: To investigate the cytotoxic effects and the potential mechanisms of crebanine N-oxide in SGC-7901 gastric adenocarcinoma cells. Methods: The cytotoxicity of crebanine N-oxide was evaluated by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide assay and cellular morphology was observed under a microscope. Cell apoptosis was determined by flow cytometry using propidium iodide staining. The expression levels of apoptotic-related proteins, cleaved caspase-3, cytochrome C, p53 and Bax, and autophagy- related proteins p62, beclin1 and LC3 were detected by Western blotting assays. Results: Crebanine N-oxide treatment significantly inhibited the proliferation of SGC-7901 cells in a dose-dependent and time- dependent manner via induction of G2-phase cell cycle arrest, apoptosis, and autophagy in SGC-7901 cells. Conclusions: Crebanine N-oxide could inhibit the growth of gastric cancer cells by promoting apoptosis and autophagy and could be used as a potential agent for treating gastric cancer.

中文翻译：

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分离自海南Stephania的天然紫花碱生物碱Crebanine N-oxide诱导人胃癌SGC-7901细胞凋亡和自噬

目的：研究肌酐N-氧化物对SGC-7901胃腺癌细胞的杀伤作用及其潜在机制。方法：用3-（4,5-二甲基-2-噻唑基）-2,5-二苯基-2-H-溴化四氮唑测定法测定肌酐N-氧化物的细胞毒性，并在显微镜下观察细胞形态。通过使用碘化丙锭染色的流式细胞术确定细胞凋亡。通过蛋白质印迹法检测凋亡相关蛋白，裂解的caspase-3，细胞色素C，p53和Bax以及自噬相关蛋白p62，beclin1和LC3的表达水平。结果：通过诱导G2期细胞周期阻滞，凋亡和自噬，肌酐N-氧化物处理以剂量依赖和时间依赖的方式显着抑制了SGC-7901细胞的增殖。结论：