FT-ICR质谱法光降解和生物降解制得的水溶性石油化合物的固相萃取和液/液萃取的分子比较,Analytical Chemistry

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FT-ICR质谱法光降解和生物降解制得的水溶性石油化合物的固相萃取和液/液萃取的分子比较
Analytical Chemistry ( IF 6.7 ) Pub Date : 2021-03-04 , DOI: 10.1021/acs.analchem.0c05230
Amy M McKenna ₁ , Huan Chen ₁ , Chad R Weisbrod ₁ , Gregory T Blakney ₁

Affiliation

我们对石油通过光降解和生物降解产生的水溶性化合物应用了两种广泛使用的萃取技术，即液/液萃取和使用具有专有非极性表面优先污染物（PPL）的苯乙烯-二乙烯基苯聚合物进行固相萃取。我们通过21 T负离子电喷雾电离傅立叶变换离子回旋共振质谱（FT-ICR MS），比较了生物降解的和光降解的水溶性有机（WSO）酸的分子组成。我们重点介绍了两种用于非生物和生物降解过程的萃取技术之间的成分差异，并鉴定了通过固/液萃取（SPE）未检测到的通过液/液萃取（LLE）鉴定出的烃类生物降解产生的已知有毒物质（环烷酸）。相同的样本。与LLE提取的化合物相比，通过SPE-PPL提取的光降解WSO化合物对应于具有更高O / C比和碳数的物种。在LLE提取物中检测到环烷酸，非芳香族羧酸的顽固类和通过油的生物降解形成的已知急性毒物（最高C₃₀和双键等效物，DBE <3），但在SPE-PPL提取物中未检测到。这表明基于油类风化过程的主要类型，LLE和SPE-PPL保留了不同的水溶性油种。

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Molecular Comparison of Solid-Phase Extraction and Liquid/Liquid Extraction of Water-Soluble Petroleum Compounds Produced through Photodegradation and Biodegradation by FT-ICR Mass Spectrometry

We apply two widely used extraction techniques, liquid/liquid extraction and solid-phase extraction with styrene–divinylbenzene polymer with a proprietary nonpolar surface priority pollutant (PPL) to water-soluble compounds generated through photodegradation and biodegradation of petroleum. We compare the molecular composition of bio- and photodegraded water-soluble organic (WSO) acids by 21 T negative-ion electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS). We highlight the compositional differences between the two extraction techniques for abiotic and biotic degradation processes and identify known toxic species (naphthenic acids) produced through hydrocarbon biodegradation identified by liquid/liquid extraction (LLE) that are not detected with solid-phase extraction (SPE) of the same sample. Photodegraded WSO compounds extracted by SPE-PPL correspond to species with higher O/C ratio and carbon number compared to LLE extracted compounds. Naphthenic acids, a recalcitrant class of nonaromatic carboxylic acids and known acute toxicants formed through biodegradation of oil, are detected in LLE extracts (up to C₃₀ and double-bond equivalents, DBE < 3) but are not detected in SPE-PPL extracts. This suggests that LLE and SPE-PPL retain different water-soluble oil species based on the dominant type of oil weathering process.

更新日期：2021-03-16

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