箱形水母Tripedalia cystophora 中的神经肽表达——对“简单”神经系统复杂性的新见解,The Journal of Comparative Neurology

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箱形水母Tripedalia cystophora 中的神经肽表达——对“简单”神经系统复杂性的新见解
The Journal of Comparative Neurology ( IF 2.3 ) Pub Date : 2021-03-04 , DOI: 10.1002/cne.25133
Sofie K D Nielsen ₁ , Thomas L Koch ₁ , Sofus H Wiisbye ₁ , Cornelis J P Grimmelikhuijzen ₁ , Anders Garm ₁

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箱形水母拥有精致的视觉系统并执行先进的视觉引导行为。然而，被认为是主要视觉处理中心的 rhopalial 神经系统 (RNS) 在携带 rhopalia 的四只眼睛中的每只眼睛中只有 1000 个神经元。我们使用针对四种推定的神经肽（T. cystophora RFamide、VWamide、RAamide 和 FRamide）的抗体进行免疫标记，检查了箱形水母Tripedalia cystophora的 RNS 的详细结构。在 RNS 中，T. cystophoraRF-、VW- 和 RAamide 抗体可染色感觉神经元、凹眼、神经细胞和茎相关神经元和巨神经元的肽特异性亚群。此外，RFamide ir+ 神经突见于表皮柄神经，而 VWamide 抗体染色胃胚柄神经。RFamide 具有最广泛的表达，包括环和桡神经、足神经丛和触手神经网。RAamide 是伞下神经网的运动神经元中推定的神经递质，而 VWamide 是神经元分化的潜在标志物，因为它存在于 rhopalia 和钟形的未分化细胞亚群中。FRamide 抗体的结果不包括在内，因为只有少数细胞被染色，并且以不可重现的方式。T. cystophora并允许我们识别神经元的特定功能组。这种识别对于理解 RNS 中的视觉处理很重要，并且能够进行实验工作，直接解决不同神经肽在视觉中的作用。

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Neuropeptide expression in the box jellyfish Tripedalia cystophora—New insights into the complexity of a “simple” nervous system

Box jellyfish have an elaborate visual system and perform advanced visually guided behaviors. However, the rhopalial nervous system (RNS), believed to be the main visual processing center, only has 1000 neurons in each of the four eye carrying rhopalia. We have examined the detailed structure of the RNS of the box jellyfish Tripedalia cystophora, using immunolabeling with antibodies raised against four putative neuropeptides (T. cystophora RFamide, VWamide, RAamide, and FRamide). In the RNS, T. cystophora RF-, VW-, and RAamide antibodies stain sensory neurons, the pit eyes, the neuropil, and peptide-specific subpopulations of stalk-associated neurons and giant neurons. Furthermore, RFamide ir+ neurites are seen in the epidermal stalk nerve, whereas VWamide antibodies stain the gastrodermal stalk nerve. RFamide has the most widespread expression including in the ring and radial nerves, the pedalium nerve plexus, and the tentacular nerve net. RAamide is the putative neurotransmitter in the motor neurons of the subumbrellar nerve net, and VWamide is a potential marker for neuronal differentiation as it is found in subpopulations of undifferentiated cells both in the rhopalia and in the bell. The results from the FRamide antibodies were not included as only few cells were stained, and in an unreproducible way. Our studies show hitherto-unseen details of the nervous system of T. cystophora and allowed us to identify specific functional groups of neurons. This identification is important for understanding visual processing in the RNS and enables experimental work, directly addressing the role of the different neuropeptides in vision.

更新日期：2021-03-04

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