Photothermal therapy (PTT) procedure is anticipated as a new generation of cancer therapy techniques. With this in mind, in this work, an effective drug-free approach was developed to kill MCF7 breast cancer cells using PTT. A novel biocompatible nanocomposite as a PTT transducer was prepared from the in situ phytosynthesis of gold nanoparticles (Au NPs) in the presence of perlite as a platform and extract of Allium Fistulosum L. as a stabilizing and reducing agent (Au/perlite NC). The common characterization techniques such as Fourier transform infrared (FT-IR), zeta potential, dynamic light scattering (DLS), X-ray diffraction (XRD), ultraviolet-visible (UV–vis), scanning electron microscopy (SEM), and energy-dispersive X-ray (EDX) were used to approve the synthesis of Au/perlite NC. The potential of the synthesized NCs on ROS generating and antioxidant activity was assessed by DPPH. In the following, the PTT efficacy of the Au/perlite NC on the destruction of MCF-7 breast cancer cells was assessed in vitro via the cell cycle, cell viability, and DAPI staining assays. The DPPH assay results showed that Au/perlite NC had a radicals scavenging capacity of about 41.47 % in 30 min. Cellular uptake results indicated a significant cell uptake after 1.5 h exposure with Au/perlite NC. Interestingly, cell death was increased dramatically by increasing irradiation time from 6 to 10 min. Cell viability assay revealed that the maximum number of cell death is around 50 % which was observed in the presence of Au/perlite NC by irradiation time of 10 min. Cell cycle results showed that the maximum amount of apoptotic cells (85 %) was observed in Au/perlite NC treatment group received laser irradiation for 10 min. The outcomes demonstrated that the Au/perlite NC can be used as a new drug-free and efficient agent for PTT of breast cancer cells without any concern cytotoxicity.

预计光热疗法（PTT）程序是新一代的癌症治疗技术。考虑到这一点，在这项工作中，开发了一种有效的无药物方法，可以使用PTT杀死MCF7乳腺癌细胞。在珍珠岩为平台和瘘孔提取物存在的条件下，通过金纳米颗粒（Au NPs）的原位植物合成制备了一种新型的生物相容性纳米复合物作为PTT换能器。L.作为稳定剂和还原剂（Au / perlite NC）。常用的表征技术，例如傅立叶变换红外（FT-IR），ζ电位，动态光散射（DLS），X射线衍射（XRD），紫外可见（UV-vis），扫描电子显微镜（SEM）和能量色散X射线（EDX）用于批准Au /珍珠岩NC的合成。通过DPPH评估了合成NCs对ROS产生和抗氧化活性的潜力。在下文中，通过细胞周期，细胞生存力和DAPI染色测定法，在体外评估了Au /珍珠岩NC的PTT对MCF-7乳腺癌细胞破坏的功效。DPPH测定结果表明，Au /珍珠岩NC在30分钟内具有约41.47％的自由基清除能力。细胞摄取结果表明1后有大量细胞摄取。用Au /珍珠岩NC曝光5小时。有趣的是，通过将照射时间从6分钟增加到10分钟，细胞死亡急剧增加。细胞活力测定显示最大细胞死亡数为约50％，这在Au /珍珠岩NC存在下通过10分钟的照射时间观察到。细胞周期结果表明，在接受激光照射10分钟的Au /珍珠岩NC处理组中，凋亡细胞的数量最多（85％）。结果表明，Au /珍珠岩NC可以用作乳腺癌细胞PTT的新型无药高效药物，而无需担心细胞毒性。细胞活力测定显示最大细胞死亡数为约50％，这在Au /珍珠岩NC存在下通过10分钟的照射时间观察到。细胞周期结果表明，金/珍珠岩NC处理组接受激光照射10分钟后，凋亡细胞的数量最多（85％）。结果表明，Au /珍珠岩NC可以用作乳腺癌细胞PTT的新型无药高效药物，而无需担心细胞毒性。细胞活力测定显示最大细胞死亡数为约50％，这在Au /珍珠岩NC存在下通过10分钟的照射时间观察到。细胞周期结果表明，金/珍珠岩NC处理组接受激光照射10分钟后，凋亡细胞的数量最多（85％）。结果表明，Au /珍珠岩NC可以用作乳腺癌细胞PTT的新型无药高效药物，而无需担心细胞毒性。