We report for the very first time self-assembly of a red color dye 7-Amino-6h-anthra[9,1-cd][1,2]thiazol-6-one (AAT),its photophysical properties and its applications in cell imaging.Interestingly, AAT show intense red colour in visible light while it shows the orange colour fluorescence under UV light @312nm.Surprisingly, when this dye was used as cell imaging agent it revealed only green fluorescence inside cells and not red. Hence, the photophysical properties of this dye was very intriguing. Further, when self-assembling properties of this dye was examined it revealed formation of tree like branched structures which appeared red both under green and red filter which was again an unexpected result..Interestingly AAT self-assembly also show morphological transition and the branched tree like structures changes to straight fibres as the solvent is changed from DMSO to THF. Hence, the results of self-assembly and cell imaging were contrary to each other and the photophysical properties of this dye is very unusual as compared to conventional dyes. Our future endeavours willaim to understand this anomalous behaviour in greater details in future through various biophysical assays.

中文翻译：

---

在细胞成像中显示绿色荧光的异常红色染料的自组装和光物理研究

我们首次报道了红色染料7-氨基-6h-蒽[9,1-cd] [1,2]噻唑-6-one（AAT）的自组装，其光物理性质及其在生物医学领域的应用。有趣的是，AAT在可见光下显示强烈的红色，而在312nm的紫外线下显示橙色的荧光。令人惊讶的是，当该染料用作细胞显像剂时，它仅显示细胞内部的绿色荧光而不显示红色。因此，这种染料的光物理性质非常吸引人。此外，当检查这种染料的自组装性能时，发现形成了树状分支结构，在绿色和红色滤光片下均显示为红色，这也是出乎意料的结果。有趣的是，随着溶剂从DMSO变为THF，AAT自组装也显示出形态转变，并且分支树状结构变为直纤维。因此，自组装和细胞成像的结果彼此相反，并且与常规染料相比，该染料的光物理性质是非常不寻常的。我们未来的努力将力争通过各种生物物理分析来更详细地了解这种异常行为。