Perfluorooctanesulfonamide (PFOSA) and 6:2 fluorotelomer sulfonate (FTSA) are widely present intermediates of per- and polyfluorinated substances (PFAS). Although detected at high concentrations in landfill leachate and groundwater, the interactions of these two compounds with plants have not been investigated much. In this work, uptake of these two PFAS intermediates at 10 and 200 μg/L by Lemna minor (common duckweed) were studied in detail. It was found that the biomass production of L. minor was not impacted negatively by PFOSA and FTSA at concentrations equal to or lower than 200 μg/L. Between these two target compounds, FTSA had much higher concentrations in L. minor when the concentrations and exposure times were the same as those for PFOSA. In addition, this compound at 200 μg/L inhibited the activities of catalase in L. minor significantly compared to the controls. This study indicates that PFOSA with low water solubility has low toxicity to L. minor, while FTSA at high concentration may accumulate in the floating plants and cause adverse effects on plant’s antioxidative defense system. Longer-term studies of L. minor with these two and other PFAS are warranted given the important role of this floating plant in the ecosystem.