TLC-bioautography amalgamated with hyphenated spectroscopy aid in precise in situ detection of secondary metabolites with pharmaceutical significance. TLC bioautography offers efficient and economical strategy in identifying compounds of interest from crude extracts. The present investigation has been focused on detection of antimicrobial metabolite from the culture broth of Nigrospora sphaerica inhabiting Croton bonplandianus Baill. The antimicrobial profiling confirmed the bioactive nigrosporalactone to possess broad-spectrum activity against test human pathogens with minimum inhibitory concentration values in the range 6.25 µg to 100 µg. The in silico studies revealed protein targets 1I01 (E. coli beta-ketoacyl reductase), 1IYL (C. albicans N-myristoyl transferase) had the highest binding score of -6.1 Kcal/mol.

结合有分光镜的TLC-生物自显影技术有助于精确原位检测次生代谢产物，具有重要的医学意义。TLC生物自显影技术可从粗提物中鉴定出目标化合物，从而提供了有效而经济的策略。目前的研究集中于从居住在巴豆bonplandianus Baill的球形黑夜蛾（Nigrospora sphaerica）的培养液中检测抗菌代谢物。抗菌谱分析证明，生物活性尼古斯波内酯具有对测试人类病原体的广谱活性，其最小抑制浓度值在6.25 µg至100 µg范围内。将在硅片的研究发现蛋白质目标1I01（大肠杆菌β-酮酰基还原酶），1IYL（白色念珠菌N-肉豆蔻酰基转移酶）的结合得分最高，为-6.1 Kcal / mol。