In vitro and in vivo analyses are closely connected, and the reciprocal relationship between the two comprises a key assumption with concern to the conducting of meaningful research. The primary purpose of in vitro analysis is to provide a solid background for in vivo and clinical study purposes. The fields of cell therapy, tissue engineering, and regenerative medicine depend upon the high quality and appropriate degree of the expansion of mesenchymal stromal cells (MSCs) under low-risk and well-defined conditions. Hence, it is necessary to determine suitable alternatives to fetal bovine serum (FBS—the laboratory gold standard) that comply with all the relevant clinical requirements and that provide the appropriate quantity of high-quality cells while preserving the required properties. Human serum (autologous and allogeneic) and blood platelet lysates and releasates are currently considered to offer promising and relatively well-accessible MSC cultivation alternatives. Our study compared the effect of heat-inactivated FBS on MSC metabolism as compared to its native form (both are used as the standard in laboratory practice) and to potential alternatives with concern to clinical application—human serum (allogeneic and autologous) or platelet releasate (PR-SRGF). The influence of the origin of the serum (fetal versus adult) was also determined. The results revealed the key impact of the heat inactivation of FBS on MSCs and the effectiveness of human sera and platelet releasates with respect to MSC behaviour (metabolic activity, proliferation, morphology, and cytokine production).

中文翻译：

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各种培养条件对人间质基质细胞代谢的影响

体外和体内分析是紧密相连的，两者之间的相互关系构成了进行有意义的研究所关注的关键假设。体外分析的主要目的是为体内提供坚实的背景和临床研究目的。细胞治疗，组织工程和再生医学的领域取决于在低风险和明确条件下间充质基质细胞（MSCs）的高质量和适当程度的扩增。因此，有必要确定适合胎牛血清的替代品（FBS，实验室金标准），该替代品应符合所有相关的临床要求，并提供适当数量的高质量细胞，同时保留所需的特性。目前认为人血清（自体和同种异体）以及血小板裂解物和释放物提供了有前途且相对容易获得的MSC培养替代品。我们的研究比较了热灭活的FBS对MSC代谢的影响（与其天然形式（均在实验室实践中用作标准））以及与临床应用有关的潜在替代物-人血清（异源和自体）或血小板释放（PR-SRGF）。还确定了血清来源（胎儿与成人）的影响。结果揭示了FBS热失活对MSC的关键影响以及人类血清和血小板释放物对MSC行为（代谢活性，增殖，形态和细胞因子产生）的有效性。