Currently, organic artificial enzymes as biocatalysts have been extensively used to construct various colorimetric sensors. However, exploiting a potential organic artificial enzyme with high catalytic efficiency still remains a challenge. To address this issue, herein, we synthesize an acridone derivative 10-benzyl-2-amino-acridone (BAA). The synthesized BAA exhibits an intrinsic visible-light-stimulated oxidase-like activity, which is capable of oxidizing various chromogenic substrates without destructive hydrogen peroxide (H₂O₂) under visible light stimulation, resulting in colored products. The reaction system can be regulated by switching light on and off, which is milder and more reliable means than others H₂O₂-dependent. The photocatalytic mechanism of BAA is investigated in detail. However, l-ascorbic acid (AA), an antioxidant generating from the acid phosphatase (ACP)-mediated hydrolysis of 2-phospho-l-ascorbic acid (AAP), is able to inhibit the catalytic activity of BAA. Based on the above properties, a facile, photo-switchable and low-cost colorimetric sensing strategy is developed for ACP detection. The linear range is 0.05–2.5 U/L (r = 0.9994), and the limit of detection (LOD) is 0.0415 U/L. Moreover, the proposed sensing system can be applied for monitoring ACP activity in practical samples, demonstrating promising applications in clinical analysis and biosensor platform.

当前，有机人工酶作为生物催化剂已被广泛用于构建各种比色传感器。然而，开发具有高催化效率的潜在有机人工酶仍然是一个挑战。为了解决这个问题，在本文中，我们合成了cri啶酮衍生物10-苄基-2-氨基-啶酮（BAA）。合成的BAA表现出固有的可见光刺激的氧化酶样活性，该活性能够在可见光刺激下氧化各种发色底物而不会破坏性的过氧化氢（H ₂ O ₂），从而产生有色产物。可以通过打开和关闭灯来调节反应系统，这是比其他H ₂ O ₂更温和，更可靠的方法。-依赖。详细研究了BAA的光催化机理。然而，1-抗坏血酸（AA）是由酸性磷酸酶（ACP）介导的2-磷酸-1-抗坏血酸（AAP）水解产生的抗氧化剂，能够抑制BAA的催化活性。基于以上特性，开发了一种用于ACP检测的简便，光开关和低成本的比色传感策略。线性范围为0.05–2.5 U / L（r = 0.9994），检测极限（LOD）为0.0415 U / L。此外，所提出的传感系统可用于监测实际样品中的ACP活性，证明其在临床分析和生物传感器平台中的应用前景广阔。