Abstract

Chitosan/gelatin (CG) composite sponges containing oxidized cellulose fibers (OF) crosslinked with tannic acid were fabricated by freeze-drying as hemostatic agents. The cellulose fibers were oxidized by 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO). Fourier transform infrared spectroscopy proved the formation of carboxyl groups on cellulose fibers. Addition of OF increased the swelling ratio of CG sponges. The amount of bleeding was measured after 10 s on hepatic trauma in a rat model. To assess the in vivo bio-absorbability of the samples, the sponges were implanted in the liver for 21 days and the volume of the repaired tissue was measured from histopathological analysis. The biocomposite sponge fabricated by addition of 30% OF suspension into CG solution showed the lowest amount of bleeding and the highest bio-absorbability after 21 days. The findings of this study indicate that the addition of OF into CG increased the clotting capability as well as the bio-absorbability of the biocomposite sponges.

Graphic abstract

中文翻译：

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新型壳聚糖/明胶/氧化纤维素海绵作为可吸收的止血剂

摘要

通过冷冻干燥作为止血剂来制备包含与鞣酸交联的氧化纤维素纤维（OF）的壳聚糖/明胶（CG）复合海绵。纤维素纤维被2,2,6,6-四甲基哌啶-1-氧基（TEMPO）氧化。傅立叶变换红外光谱证明了纤维素纤维上羧基的形成。OF的添加增加了CG海绵的溶胀率。在大鼠模型中肝外伤10秒钟后测量出血量。为了评估样品的体内生物吸收能力，将海绵植入肝脏中21天，然后根据组织病理学分析测量修复组织的体积。通过在CG溶液中添加30％OF悬浮液制成的生物复合海绵在21天后显示出最低的出血量和最高的生物吸收性。

图形摘要