植物氨基酸转运蛋白介导的聚琥珀酰亚胺纳米载体在氟啶酮中的韧皮部递送对香蕉枯萎病的控制作用,Journal of Agricultural and Food Chemistry

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植物氨基酸转运蛋白介导的聚琥珀酰亚胺纳米载体在氟啶酮中的韧皮部递送对香蕉枯萎病的控制作用
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2021-02-25 , DOI: 10.1021/acs.jafc.0c07028
Hanxiang Wu _{1,

2} , Pengtong Hu _{2,

3} , Ye Xu _{2,

3} , Chunxia Xiao ₂ , Zhibin Chen ₂ , Xiaojing Liu ₂ , Jinliang Jia _{1,

2,

3,

4} , Hanhong Xu _{1,

2,

4}

Affiliation

枯萎病对香蕉的全球生产构成了严重威胁。将杀真菌剂定向递送至香蕉韧皮部组织可能为控制这种难以治疗的血管疾病提供新的希望。在本研究中，制备了氟尿酮（FLU）负载的甘氨酸甲酯共轭聚琥珀酰亚胺纳米颗粒（PGA），负载效率（LE）为27.9％。所获得的纳米颗粒（FLU @ PGA）表现出pH敏感的控制释放，特别是在植物韧皮部的碱性pH下。体内在盆栽香蕉中进行的实验表明，FLU @ PGA可以在叶面施用后向香蕉根茎和根部向下递送FLU，从而将疾病严重程度降低50.4％。韧皮部转运研究表明，FLU @ PGA的韧皮部负载参与了器官水平（蓖麻籽幼苗）的主动转运机制。荧光素5-异硫氰酸酯尸胺标记的PGA纳米载体的观察表明，它们可以被叶肉细胞吸收并通过增生途径被加载到血管组织中。此外，观察到FLU @ PGA与植物氨基酸转运蛋白AtLHT1的相互作用在细胞水平上增强了跨膜摄取（非洲爪蟾）卵母细胞）。这些结果表明转运蛋白介导的纳米载体以韧皮部为目标的杀菌剂的递送可能是香蕉香蕉枯萎病管理的有希望的新策略。

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Phloem Delivery of Fludioxonil by Plant Amino Acid Transporter-Mediated Polysuccinimide Nanocarriers for Controlling Fusarium Wilt in Banana

Fusarium wilt disease poses a serious threat to the global production of bananas. The targeted delivery of fungicides to banana phloem tissues may offer new hope for controlling this hard-to-treat vascular disease. In this study, fludioxonil (FLU)-loaded glycine methyl ester-conjugated polysuccinimide nanoparticles (PGA) were prepared with a loading efficiency (LE) of 27.9%. The obtained nanoparticles (FLU@PGA) exhibited pH-sensitive controlled release, specifically under an alkaline pH in plant phloem. In vivo experiments in potted bananas demonstrated that FLU@PGA can achieve the downward delivery of FLU to banana rhizomes and roots after foliar application, reducing disease severity by 50.4%. The phloem transport studies showed that the phloem loading of FLU@PGA was involved in an active transport mechanism at the organ level (castor bean seedlings). The observation of fluorescein-5-isothiocyanate cadaverine-labeled PGA nanocarriers showed that they could be absorbed by mesophyll cells and loaded into vascular tissues through the symplastic pathway. Furthermore, the interaction of FLU@PGA with the plant amino acid transporter AtLHT1 was observed to enhance transmembrane uptake at the cellular level (Xenopus oocytes). These results suggested that the phloem-targeted delivery of fungicide by transporter-mediated nanocarriers could be a promising new strategy for the management of Fusarium wilt in bananas.

更新日期：2021-03-10

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