Ginsenoside Rb1 is the main predominant component in Panax species. In this study, an eco-friendly and convenient preparation method for ginsenoside CK has been established, and five strains of β-glucosidase-producing microorganisms were screened out from the soil of a Panax notoginseng planting field using Esculin-R2A agar. Aspergillus niger XD101 showed that it has excellent biocatalytic activity for ginsenosides; one of the isolates can convert ginsenoside Rb1 to CK using extracellular enzyme from the mycelium. Mycelia of A. niger were cultivated in wheat bran media at 30 °C for 11 days. By the removal of mycelia from cultured broth, enzyme salt fractionation by ammonium sulfate (70%, v/v) precipitation, and dialysis, sequentially, crude enzyme preparations from fermentation liquid supernatant were obtained. The enzymatic transformed Rb1 as the following pathways: Rb1→Rd→F2→CK. The optimized reaction conditions are at reaction time of 72 h, in the range of pH 4–5, and temperature of 50–60 °C. Active minor ginsenosides can be obtained by a specific bioconversion via A. niger XD101 producing the ginsenoside-hydrolyzing β-glucosidase. In addition, the crude enzyme can be resulted in producing ginsenoside CK via conversion of ginsenoside Rb1 at high conversion yield (94.4%). FDA generally regarded, A.niger as safe microorganism. Therefore, these results indicate that A. niger XD10 may provide an alternative method to prepare ginsenoside CK without food safety issues in the pharmaceutical industry.

人参皂苷Rb1是在主主要成分三七品种。本研究建立了一种环保、简便的人参皂苷CK制备方法，利用七七-R2A琼脂从三七种植地土壤中筛选出5株产β-葡萄糖苷酶微生物。黑曲霉XD101表明其对人参皂苷具有优良的生物催化活性；其中一个分离株可以使用来自菌丝体的胞外酶将人参皂苷 Rb1 转化为 CK。黑曲霉菌丝体在麦麸培养基中于 30°C 培养 11 天。通过从培养液中去除菌丝体，硫酸铵（70%，v/v）沉淀酶盐分馏，透析，依次从发酵液上清液中获得粗酶制剂。酶促转化Rb1的途径如下：Rb1→Rd→F2→CK。优化的反应条件是反应时间为 72 h，pH 范围为 4-5，温度为 50-60 °C。活性次要人参皂苷可以通过特定的生物转化获得，通过黑曲霉XD101 产生人参皂苷水解 β-葡萄糖苷酶。此外，粗酶可以通过以高转化率 (94.4%) 转化人参皂苷 Rb1 来生产人参皂苷 CK。FDA普遍认为，A.niger作为安全微生物。因此，这些结果表明，A. niger XD10 可以提供一种替代方法来制备人参皂苷 CK，而不会出现制药行业的食品安全问题。