The proximity of the G-quadruplex to hemin impacts the intrinsic DNAzyme activity in mitochondria,Chemical Communications

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The proximity of the G-quadruplex to hemin impacts the intrinsic DNAzyme activity in mitochondria
Chemical Communications ( IF 4.3 ) Pub Date : 2021-2-9 , DOI: 10.1039/d0cc08316j
Jiayu Zhang ₁ , Xiaofang Song , Miaoren Xia , Yanan Xue , Mengxue Zhou , Lifo Ruan , Huiru Lu , Jun Chen , Dongqi Wang , Zhifang Chai , Yi Hu

Affiliation

The DNAzyme activity of G-quadruplex/hemin in mitochondria has not been characterized. Herein, we report an unexpected difference in the DNAzyme activity between IN VITRO assays and in mitochondria. Molecular dynamic simulations illustrate how the interaction of the G-quadruplex with hemin may modulate the DNAzyme activity. These results might facilitate a better understanding of the catalytic mechanism of the DNAzyme and help the rational design of stable and active DNAzymes suitable for intracellular catalysis.

中文翻译：

G-四链体与血红素的接近性影响线粒体中固有的DNAzyme活性

线粒体中的G-四链体/血红素的DNAzyme活性尚未被表征。在本文中，我们报道了在体外测定与线粒体之间的DNAzyme活性意外差异。分子动力学模拟说明了G-四链体与血红素的相互作用如何调节DNAzyme的活性。这些结果可能有助于更好地了解DNAzyme的催化机制，并有助于合理设计适合细胞内催化作用的稳定和活性DNAzyme。

更新日期：2021-02-24

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