Abstract

Four new compounds (derriscandenon D (1), E (2), F (3), G (4)) and six known isoflavones (warangalone (5), millewanin E (6), rhynedlin A (7), 6,8-diprenylgenistein (8), isolupalbigenin (9), isoscandinone (10)) were isolated from the acetone extract of the branches of Derris scandens. These compounds were assayed for cell viability using the human lung carcinoma cell line A549, colorectal carcinoma cell line Colo205, epidermoid carcinoma cell line KB, the human acute lymphoblastic leukaemia cell line NALM-6, and human dermal fibroblasts. Compounds 2 and 3 significantly decreased the viability of KB cells, with IC₅₀ values of 2.7 and 12.9 µM, respectively. In addition, compounds 2 and 3 reduced the mitochondrial membrane potential in KB cells. Compounds 2 and 3 strongly down-regulated the cell viability of cell lines KB and NALM-6, achieving IC₅₀ values of 2.7 and 0.9 µM, respectively, compared with the positive control staurosporine at 1.25 and 0.01 µM, respectively.

摘要

四种新化合物（derriscandenon D ( 1 )、E ( 2 )、F ( 3 )、G ( 4 )）和六种已知异黄酮（warangalone ( 5 )、millewanin E ( 6 )、rhynedlin A ( 7 )、6,8 -diprenylgenistein ( 8 ), isolupalbigenin ( 9 ), isoscandinone ( 10 )) 是从Derris scandens分支的丙酮提取物中分离出来的。使用人肺癌细胞系 A549、结肠直肠癌细胞系 Colo205、表皮样癌细胞系 KB、人急性淋巴细胞白血病细胞系 NALM-6 和人真皮成纤维细胞测定这些化合物的细胞活力。化合物2和3显着降低了 KB 细胞的活力，IC ₅₀值分别为 2.7 和 12.9 µM。此外，化合物2和3降低了 KB 细胞的线粒体膜电位。化合物2和3强烈下调细胞系 KB 和 NALM-6 的细胞活力，与分别为 1.25 和 0.01 μM 的阳性对照星形孢菌素相比， IC _{50值分别为 2.7 和 0.9 μM。}