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Naphthalimide-4-(4-nitrophenyl)thiosemicarbazide: A Fluorescent Probe for Simultaneous Monitoring of Viscosity and Nitric Oxide in Living Cells
Analytical Chemistry ( IF 6.7 ) Pub Date : 2021-02-22 , DOI: 10.1021/acs.analchem.0c04019
Su Jung Kim 1 , Sun Young Park 1 , Shin A Yoon 1 , Changshin Kim 2 , Chulhun Kang 2 , Min Hee Lee 1
Analytical Chemistry ( IF 6.7 ) Pub Date : 2021-02-22 , DOI: 10.1021/acs.analchem.0c04019
Su Jung Kim 1 , Sun Young Park 1 , Shin A Yoon 1 , Changshin Kim 2 , Chulhun Kang 2 , Min Hee Lee 1
Affiliation
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Intracellular viscosity is a physicochemical factor that determines the outcomes of various biological processes, while nitric oxide (NO) is an essential signaling molecule that controls many cellular processes, including oxidative stress. Anticipating that both may be interrelated with a variety of pathologies, their simultaneous measurement would be highly valuable for the investigation of the pathological condition of cells. However, the development of a sensor for such simultaneous detection has not been attempted yet. Herein, we present the synthesis of naphthalimide-4-(4-nitrophenyl)thiosemicarbazide, probe 1, and its application to living cells under conditions of lipopolysaccharide or nystatin treatment, adopted as oxidative stress and altered intracellular viscosity models, respectively. The probe showed increased fluorescence in response to elevation of viscosity and NO levels at 470 and 550 nm, respectively, in the solution studies. When the probe was used for a confocal microscopic study of HeLa cells under stressed conditions, simultaneous monitoring of viscosity and NO level elevations was possible through fluorescence imaging using band-pass filters of 420–475 and 505–600 nm, respectively, upon excitation at a wavelength of 405 nm. Interestingly, both the cellular viscosity and NO levels increased together under lipopolysaccharide or nystatin treatment. Therefore, we suggest that probe 1 is a fluorescent chemical probe that enables the monitoring of alterations in intracellular viscosity and NO levels in living cells, which would be valuable in studies of various cellular damage models.
中文翻译:
萘二甲酰亚胺-4-(4-硝基苯基)硫代氨基脲:同时监测活细胞中粘度和一氧化氮的荧光探针
细胞内粘度是决定各种生物过程结果的物理化学因素,而一氧化氮(NO)是控制许多细胞过程(包括氧化应激)的重要信号分子。预期两者可能与多种病理相关,因此它们的同时测量对于研究细胞的病理状况将非常有价值。但是,尚未尝试开发用于这种同时检测的传感器。在这里,我们提出萘二甲酰亚胺-4-(4-硝基苯基)硫代氨基脲的合成,探针1,并将其应用于脂多糖或制霉菌素处理条件下的活细胞中,分别用作氧化应激和改变的细胞内粘度模型。在溶液研究中,该探针分别显示出响应于470和550 nm处粘度和NO水平升高的荧光增强。当将探针用于在压力条件下对HeLa细胞的共聚焦显微镜研究时,通过分别在420-475和505-600 nm的带通滤光片进行荧光成像,可以在荧光激发下同时监测粘度和NO水平升高。 405 nm的波长。有趣的是,在脂多糖或制霉菌素处理下,细胞粘度和NO水平都一起增加。因此,我们建议探针1 是一种荧光化学探针,能够监测活细胞中细胞内粘度和NO水平的变化,这对各种细胞损伤模型的研究非常有价值。
更新日期:2021-03-16
中文翻译:
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萘二甲酰亚胺-4-(4-硝基苯基)硫代氨基脲:同时监测活细胞中粘度和一氧化氮的荧光探针
细胞内粘度是决定各种生物过程结果的物理化学因素,而一氧化氮(NO)是控制许多细胞过程(包括氧化应激)的重要信号分子。预期两者可能与多种病理相关,因此它们的同时测量对于研究细胞的病理状况将非常有价值。但是,尚未尝试开发用于这种同时检测的传感器。在这里,我们提出萘二甲酰亚胺-4-(4-硝基苯基)硫代氨基脲的合成,探针1,并将其应用于脂多糖或制霉菌素处理条件下的活细胞中,分别用作氧化应激和改变的细胞内粘度模型。在溶液研究中,该探针分别显示出响应于470和550 nm处粘度和NO水平升高的荧光增强。当将探针用于在压力条件下对HeLa细胞的共聚焦显微镜研究时,通过分别在420-475和505-600 nm的带通滤光片进行荧光成像,可以在荧光激发下同时监测粘度和NO水平升高。 405 nm的波长。有趣的是,在脂多糖或制霉菌素处理下,细胞粘度和NO水平都一起增加。因此,我们建议探针1 是一种荧光化学探针,能够监测活细胞中细胞内粘度和NO水平的变化,这对各种细胞损伤模型的研究非常有价值。