This study proposed a strategy to maximize the hydrogen yield by reusing feedstock of Arundo donax L. For this purpose, a successive 4-batch photo-fermentative hydrogen production (PFHP) was carried out to test the strategy. About 50% of total hydrogen yield was additionally obtained by reusing the Arundo donax L for successive 4 times in comparison to single 1st batch (161.4 mL/U. cell dry weight). In addition to the highest hydrogen yield, the maximum hydrogen production rate (6.0 mL/U. cell dry weight /h), and the highest volatile fatty acids (VFAs) concentration (32 mM) were also obtained from the 1st batch, while the 2nd batch gave the maximum substrate conversion efficiency (96.5%). Moreover, a positive relationship between the sum of acetic and butyric acids with hydrogen yields was observed. This strategy would help in enhancing hydrogen yield that coupled with cost reduction for biohydrogen production.

这项研究提出了一种策略，通过重复利用Arundo donax L的原料来最大程度地提高氢气产量。为此，连续进行了4批光发酵制氢（PFHP），以测试该策略。通过重复使用Arundo donax，还可获得约50％的总氢产量。与第一个批次（161.4 mL / U细胞干重）相比，L连续4次。除了最高的氢气产量外，第一批产品还获得了最高的氢气生产率（6.0 mL / U。细胞干重/ h）和最高的挥发性脂肪酸（VFAs）浓度（32 mM）。第2批产生最大的底物转化效率（96.5％）。而且，观察到乙酸和丁酸的总和与氢产率之间呈正相关。该策略将有助于提高氢气产量，同时降低生物氢气生产的成本。